AB258122

Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate

Be the first to review this product! 리뷰 제출

(0 출판물)

PLCB3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2 and Insertion of the selection cassette in exon2.

대체 명칭 보기

PLCB3, PLC-beta-3, Phosphoinositide phospholipase C-beta-3, Phospholipase C-beta-3

4 이미지

Lab

Western blot - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate, 20 ug
Lane 2 : PLCB3 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate, 20 ug

Lanes 1 - 2 : Merged signal (red and green). Green - ab124735 observed at 150 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab124735 was shown to react with Phospholipase C beta 3/PLCB3 in wild-type HeLa cells in Western blot with loss of signal observed in PLCB3 knockout cell line ab266034 (PLCB3 knockout cell lysate ab258122). Wild-type HeLa and PLCB3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab124735 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (<a href='/ko/products/primary-antibodies/phospholipase-c-beta-3-plcb3-antibody-epr5951-ab124735'>ab124735</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

PLCB3 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell line (<a href='/ko/products/cell-lines/human-plcb3-phospholipase-c-beta-3-knockout-hela-cell-line-ab266034'>ab266034</a>)

Predicted band size: 139 kDa

Observed band size: 150 kDa

false

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Sanger seq

Unknown

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Allele-1 : 4 bp deletion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Allele-2 : Insertion of the selection cassette in exon2

Sanger seq

Unknown

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Allele-3 : Insertion of the selection cassette in exon2

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2 and Insertion of the selection cassette in exon2.

질병

Adenocarcinoma

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style

left-column

제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258122-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258122-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human PLCB3 knockout HeLa cell lysate", "number":"AB258122-CMP02", "productcode":"" } ] } } }

style

left-column

특성 및 보관 정보

유전자명

PLCB3

유전자 편집 종류

Knockout

유전자 편집 방식

CRISPR technology

Knockout 검증

Sanger Sequencing, Western blot

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C beta 3 often called PLCB3 plays an important role in cellular signal transduction. This enzyme exists as part of the phospholipase C family enhances the conversion of phosphatidylinositol 45-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol triphosphate (IP3). These molecules act as secondary messengers and are important to cellular responses. PLCB3 has a molecular mass of approximately 152 kDa. It exhibits significant expression in various tissues including the brain and heart among others where it regulates numerous physiological processes.

Biological function summary

The activation of PLCB3 influences intracellular calcium levels by producing IP3 contributing to calcium signaling pathways. As part of its biological role PLCB3 contributes to a dynamic signaling complex interacting with receptors like G-protein-coupled receptors (GPCRs) and affecting downstream signaling. This enzyme actively participates in regulating cellular processes such as proliferation and differentiation by mediating signal transduction pathways.

Pathways

PLCB3 integrates into the calcium signaling and phosphatidylinositol signaling pathways where it functions through interaction with G alpha q proteins. The activation of PLCB3 leads to the release of calcium ions from the endoplasmic reticulum a process important for many cellular functions. In these pathways PLCB3 shares interactions with other related proteins like protein kinase C (PKC) which further modulates cellular responses.

Dysregulation of PLCB3 activity has links to several pathophysiological conditions including cardiovascular diseases and cancer. Abnormal PLCB3 signaling correlates with irregular calcium homeostasis impacting cardiac functions and promoting oncogenic transformations. Interactions between PLCB3 and proteins such as GPCRs highlight their importance in developing therapeutic targets for treating these diseases.

style

left-column

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style

left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style

left-column

제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate

Western blot - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

주요 정보

세포 유형

Species or organism

조직

Knockout 검증

Mutation 정보

질병

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

유전자명

유전자 편집 종류

유전자 편집 방식

Knockout 검증

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

품질 관리

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

제품 프로토콜

Product promise