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AB258593

Human PMAIP1 (Noxa) knockout A549 cell lysate

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PMAIP1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp deletion in exon1.
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Sanger Sequencing - Human PMAIP1 (Noxa) knockout A549 cell lysate (AB258593)
  • Sanger seq

Unknown

Sanger Sequencing - Human PMAIP1 (Noxa) knockout A549 cell lysate (AB258593)

Allele-1 : 2 bp deletion in exon1

Sanger Sequencing - Human PMAIP1 (Noxa) knockout A549 cell lysate (AB258593)
  • Sanger seq

Unknown

Sanger Sequencing - Human PMAIP1 (Noxa) knockout A549 cell lysate (AB258593)

Allele-2 : 1 bp insertion in exon1

주요 정보

세포 유형

A549

Species or organism

Human

조직

Lung

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp deletion in exon1.

질병

Carcinoma

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보완 제품

Primary Antibodies

AB13654

Anti-Noxa antibody [114C307]

Western blot - Anti-Noxa antibody [114C307] (AB13654)

  • 4
  • 6
Proteins & Peptides

AB152618

Recombinant Human Noxa protein

SDS-PAGE - Recombinant Human Noxa protein (AB152618)

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  • 0
Cell Lines & Lysates

AB94149

Tyrosinase overexpression 293T lysate (whole cell)

Western blot - Tyrosinase overexpression 293T lysate (whole cell) (AB94149)

  • 0
  • 0
Primary Antibodies

AB98861

Anti-PBA1 antibody

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  • 0
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258593-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PMAIP1 knockout A549 cell lysate", "number":"AB258593-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type A549 cell lysate", "number":"AB258593-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
PMAIP1
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Noxa also known as PMAIP1 is a pro-apoptotic member of the Bcl-2 protein family. It has a molecular weight of approximately 20 kDa. Noxa commonly expresses in a number of tissues including lymphoid and myeloid lineages. It functions by interacting with other Bcl-2 family proteins like Mcl-1 promoting apoptosis through binding and neutralization of anti-apoptotic proteins. This interaction facilitates the release of cytochrome c from the mitochondria triggering caspase activation and subsequent apoptosis.
Biological function summary

Noxa plays a role in the regulation of programmed cell death including being part of multiprotein complexes within the mitochondria. Noxa's expression is upregulated in response to various cellular stress signals. These stresses include DNA damage and hypoxia where it acts to mediate apoptosis preventing the survival of cells with potential mutations. Its role in modulating apoptosis makes it key in maintaining cellular homeostasis.

Pathways

Noxa is involved in significant apoptotic and cellular stress response pathways. It forms part of the intrinsic apoptosis pathway which is tightly regulated by Bcl-2 family members like BAK and BAX. Noxa's ability to neutralize Mcl-1 allows the activation of these proteins committing the cell to apoptosis. It also participates in the p53 signaling pathway which is important for the cellular response to DNA damage wherein p53 directly transactivates Noxa.

Noxa is important in cancer and autoimmune syndromes. In cancer its expression often correlates with the sensitivity of cancer cells to chemotherapy as its role in apoptosis can enhance the elimination of cancerous cells. Mutations in p53 that impair its function can lead to decreased Noxa expression and apoptosis contributing to cancer progression. In autoimmune disorders aberrant regulation of apoptosis sometimes involving Noxa and its interaction with Bcl-2 proteins can result in premature cell death or survival of autoreactive cells exacerbating the disease.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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제품 프로토콜

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Product promise

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