Human POLM (DNA polymerase mu) knockout HeLa cell lysate

2 이미지

• WB

Lab

Western blot - Human POLM (DNA polymerase mu) knockout HeLa cell lysate (AB258127)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : POLM knockout HeLa cell lysate (20 μg)

Lane 3 : HEK-293 cell lysate (20 μg)

Lane 4 : Daudi cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab157465 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.

ab157465 Anti-DNA polymerase mu antibody [EPR10470(B)] was shown to specifically react with POLM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265557 (knockout cell lysate ab258127) was used. Wild-type and POLM knockout samples were subjected to SDS-PAGE. ab157465 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (<a href='/ko/products/primary-antibodies/dna-polymerase-mu-antibody-epr10470b-ab157465'>ab157465</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

POLM knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human POLM (DNA polymerase mu) knockout HeLa cell line (<a href='/ko/products/cell-lines/human-polm-dna-polymerase-mu-knockout-hela-cell-line-ab265557'>ab265557</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 52 kDa

false

• Sanger seq

Unknown

Sanger Sequencing - Human POLM (DNA polymerase mu) knockout HeLa cell lysate (AB258127)

Homozygous : 1 bp insertion in exon4