AB257042

Human PRKCD (PKC delta) knockout HEK-293T cell lysate

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PRKCD KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2.

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Western blot - Human PRKCD (PKC delta) knockout HEK-293T cell lysate (AB257042)

Lane 1 : Wild-type HEK-293T cell lysate 20 ug
Lane 2 : PRKCD knockout HEK-293T cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab182126 observed at 78 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.#ab182126 was shown to react with PKC in wild-type HeLa cells in western blot. The bands observed in PRKCD knockout cell line ab266143 (PRKCD knockout cell lysate ab257042) below 78kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and PRKCD knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab182126 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4^°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PKC delta antibody [EPR17075] (<a href='/ko/products/primary-antibodies/pkc-delta-antibody-epr17075-ab182126'>ab182126</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PRKCD knockout HEK-293T cell lysate at 20 µg

Predicted band size: 78 kDa

Observed band size: 78 kDa

false

Sanger Sequencing - Human PRKCD (PKC delta) knockout HEK-293T cell lysate (AB257042)

Sanger seq

Unknown

Sanger Sequencing - Human PRKCD (PKC delta) knockout HEK-293T cell lysate (AB257042)

Allele-1 : 2 bp deletion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human PRKCD (PKC delta) knockout HEK-293T cell lysate (AB257042)

Allele-2 : 1 bp insertion in exon2

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257042-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257042-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human PRKCD knockout HEK293T cell lysate", "number":"AB257042-CMP01", "productcode":"" } ] } } }

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특성 및 보관 정보

유전자명

PRKCD

유전자 편집 종류

Knockout

유전자 편집 방식

CRISPR technology

Knockout 검증

Sanger Sequencing

품질 관리 - STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C delta (PKC delta) often called PKCδ belongs to the novel PKC subfamily. It weighs approximately 78 kDa and functions in various cellular processes. This enzyme exhibits serine/threonine kinase activity and is expressed across numerous tissues including the brain heart and lymphoid organs. PKC delta activation mainly relies on diacylglycerol and phospholipids though it does not require calcium for activation. This target has multiple known phosphorylation sites such as delta 1485 and delta 647 that modulate its enzymatic activity and localization.

Biological function summary

PKC delta influences cell growth apoptosis and differentiation through its kinase activity. It acts as an important regulator in the signaling pathways that control these processes. PKC delta is not a part of a traditional complex but interacts with several proteins influencing signaling cascades. In cellular contexts PKC delta positively influences tumor necrosis factor-related pathways and also initiates apoptosis in response to DNA damage.

Pathways

PKC delta plays an essential role in the regulation of mitogen-activated protein kinase (MAPK) and Janus kinase (JAK) pathways. Within the MAPK pathway PKC delta interacts with proteins like c-Jun N-terminal kinase (JNK) facilitating stress-responsive pathways related to cell death and survival. In the JAK pathway its regulation can impact cytokine signaling and immune responses. PKC delta's function within these pathways positions it as an important mediator and influencer of diverse cellular responses.

PKC delta has significant implications for cancer and cardiovascular diseases. PKC delta promotes tumor cell proliferation in some cancers by altering cell cycle regulation and resist apoptosis. In cardiovascular disorders this protein mediates cardiac hypertrophy and heart failure through its influence on myocyte apoptosis and fibrosis. PKC delta’s interaction with specific proteins like Bcl-2 in cancer and troponin in cardiac conditions highlights its role in pathophysiological processes making it a significant target for therapeutic intervention.

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PRKCD (PKC delta) knockout HEK-293T cell lysate

Western blot - Human PRKCD (PKC delta) knockout HEK-293T cell lysate (AB257042)

Sanger Sequencing - Human PRKCD (PKC delta) knockout HEK-293T cell lysate (AB257042)

Sanger Sequencing - Human PRKCD (PKC delta) knockout HEK-293T cell lysate (AB257042)

주요 정보

세포 유형

Species or organism

조직

Knockout 검증

Mutation 정보

보완 제품

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

유전자명

유전자 편집 종류

유전자 편집 방식

Knockout 검증

품질 관리 - STR analysis

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

Cell culture

Biosafety level

Adherent/suspension

Gender

제품 프로토콜

Product promise