Human PSD95 knockout SH-SY5Y cell lysate

• WB

Lab

Western blot - Human PSD95 knockout SH-SY5Y cell lysate (AB280102)

Lane 1 : Wild-type SH-SY5Y cell lysate 20 μg
Lane 2 : DLG4 knockout SH-SY5Y cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
Lane 4 : HL-60 cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab238135 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab238135 was shown to react with PSD95 in wild-type SH-SY5Y cells in Western blot with loss of signal observed in DLG4 knockout cell line ab280043 (DLG4 knockout cell lysate ab280102). Wild-type SH-SY5Y and DLG4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab238135 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 ^°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (<a href='/ko/products/primary-antibodies/psd95-antibody-epr23124-118-synaptic-marker-ab238135'>ab238135</a>) at 1/2000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 2:

DLG4 knockout SH-SY5Y cell lysate at 20 µg

Lane 2:

Western blot - Human PSD95 knockout SH-SY5Y cell line (<a href='/ko/products/cell-lines/human-psd95-knockout-sh-sy5y-cell-line-ab280043'>ab280043</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HL-60 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 80 kDa

Observed band size: 75 kDa

false

• Sanger seq

Supplier Data

Sanger Sequencing - Human PSD95 knockout SH-SY5Y cell lysate (AB280102)

Human PSD95 KO in SH-SY5Y Cells with 40 bp Deletion in Exon 5