AB258145

Human PSMB10 (MECL1) knockout HeLa cell lysate

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PSMB10 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

대체 명칭 보기

Proteasome MECl-1, Proteasome subunit beta type-10, LMP10, PSMB10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Low molecular mass protein 10, MECL1, Proteasome subunit beta-2i

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Lab

Western blot - Human PSMB10 (MECL1) knockout HeLa cell lysate (AB258145)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : PSMB10 knockout HeLa cell lysate (20 ug)
Lane 3 : Daudi cell lysate (20 ug)
Lane 4 : Raji cell lysate (20 ug)

ab183506 was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264923 (knockout cell lysate ab258145) was used. Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE. ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (<a href='/ko/products/primary-antibodies/psmb10-mecl1-antibody-epr14902-ab183506'>ab183506</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PSMB10 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PSMB10 (MECL1) knockout HeLa cell line (<a href='/ko/products/cell-lines/human-psmb10-mecl1-knockout-hela-cell-line-ab264923'>ab264923</a>)

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

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Sanger Sequencing - Human PSMB10 (MECL1) knockout HeLa cell lysate (AB258145)

Sanger seq

Unknown

Sanger Sequencing - Human PSMB10 (MECL1) knockout HeLa cell lysate (AB258145)

Homozygous : 1 bp deletion in exon 1

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

질병

Adenocarcinoma

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258145-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PSMB10 knockout HeLa cell lysate", "number":"AB258145-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258145-CMP01", "productcode":"" } ] } } }

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특성 및 보관 정보

유전자명

PSMB10

유전자 편집 종류

Knockout

유전자 편집 방식

CRISPR technology

Knockout 검증

Sanger Sequencing, Western blot

Zygosity

Homozygous

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PSMB10 also known as MECL1 is a proteasome component involved in protein degradation. This protein has a molecular mass of approximately 29 kDa. PSMB10 is expressed in various tissues with notable expression in the immune system. As a beta subunit of the 20S proteasome MECL1 has a role in cleaving peptides at specific sites contributing to the breakdown of ubiquitinated proteins.

Biological function summary

PSMB10/MECL1 plays a significant role in immune responses. It forms part of the immunoproteasome a variant of the proteasome complex which enhances the generation of antigenic peptides presented on MHC class I molecules. This function is essential for effective immune surveillance and initializing adaptive immunity. In particular PSMB10's influence on antigen processing positions it as an important player in modulating immune system activity.

Pathways

PSMB10/MECL1 participates in pathways associated with protein catabolism and immune signaling. It is involved in the ubiquitin-proteasome pathway which is critical for maintaining cellular protein balance. The function of PSMB10 within the immunoproteasome links it to immune signaling pathways. It has associations with other 20S proteasome subunits such as PSMB8 and PSMB9 which also contribute to the formation of the immunoproteasome complex.

PSMB10/MECL1 has connections to autoimmune diseases and cancer. In autoimmune diseases the altered function of PSMB10 within the immunoproteasome can lead to improper antigen presentation potentially triggering excessive immune responses. In oncology aberrant expression of immunoproteasome subunits including PSMB10 has implications in tumor immune evasion. The interplay with proteins like PSMB9 in these contexts suggests an intricate network affecting disease progression and immune regulation.

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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PSMB10 (MECL1) knockout HeLa cell lysate

Western blot - Human PSMB10 (MECL1) knockout HeLa cell lysate (AB258145)

Sanger Sequencing - Human PSMB10 (MECL1) knockout HeLa cell lysate (AB258145)

주요 정보

세포 유형

Species or organism

조직

Knockout 검증

Mutation 정보

질병

보완 제품

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Cell Lines & Lysates

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

유전자명

유전자 편집 종류

유전자 편집 방식

Knockout 검증

Zygosity

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

품질 관리

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

제품 프로토콜

Product promise