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AB261686

Human PVR (Poliovirus Receptor) knockout A549 cell lysate

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PVR KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 11 bp deletion Frameshift = 100%.
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Western blot - Human PVR (Poliovirus Receptor) knockout A549 cell lysate (AB261686)
  • WB

Lab

Western blot - Human PVR (Poliovirus Receptor) knockout A549 cell lysate (AB261686)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : PVR knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : U87-MG whole cell lysate 20 ug

Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab205304 observed at 70 kDa (ab205304), 60-80 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab205304 was shown to specifically react with Poliovirus Receptor in wild-type A549 cells as signal was lost in PVR knockout cell line ab261877 (knockout cell lysate ab261686). Wild-type and PVR knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab205304 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (<a href='/ko/products/primary-antibodies/poliovirus-receptor-pvr-antibody-epr17302-ab205304'>ab205304</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

PVR knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PVR (Poliovirus Receptor) knockout A549 cell line (<a href='/ko/products/cell-lines/human-pvr-poliovirus-receptor-knockout-a549-cell-line-ab261877'>ab261877</a>)

Lane 3:

U87-MG whole cell lysate at 20 µg

Lane 4:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Predicted band size: 45 kDa

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Next Generation Sequencing - Human PVR (Poliovirus Receptor) knockout A549 cell lysate (AB261686)
  • NGS

Supplier Data

Next Generation Sequencing - Human PVR (Poliovirus Receptor) knockout A549 cell lysate (AB261686)

Knockout achieved by CRISPR/Cas9; X = 11 bp deletion; Frameshift = 100%

주요 정보

세포 유형

A549

Species or organism

Human

조직

Lung

Knockout 검증

Next Generation Sequencing,Western blot

Mutation 정보

Knockout achieved by CRISPR/Cas9 X = 11 bp deletion Frameshift = 100%

질병

Carcinoma

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보완 제품

Primary Antibodies

AB199093

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB199093)

  • 0
  • 0
Secondary Antibodies

AB6785

Goat Anti-Mouse IgG H&L (FITC)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (AB6785)

  • 5
  • 1
Secondary Antibodies

AB131368

Anti-mouse IgG for IP (HRP)

VeriBlot

Western blot - Anti-mouse IgG for IP (HRP) (AB131368)

  • 3
  • 3
Secondary Antibodies

AB96907

Goat Anti-Human IgG H&L (DyLight® 488)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab261686-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type A549 cell lysate", "number":"AB261686-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human PVR knockout A549 cell lysate", "number":"AB261686-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
PVR
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Next Generation Sequencing, Western blot
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Poliovirus Receptor (PVR) also known as CD155 is a cell surface glycoprotein with a molecular mass of approximately 70 kDa. This receptor is expressed on a variety of cell types including epithelial and endothelial cells immune cells and fibroblasts. Another name for PVR is Necl-5 which falls under the nectin-like molecule family. It acts as an adhesion molecule and contributes to cellular signaling and junctional complexes. The expression of PVR is widespread across multiple tissues but it exhibits stronger expression in areas such as the skin and the gastrointestinal tract.
Biological function summary

The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.

Pathways

PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.

PVR relates to conditions such as cancer and viral infection. Its overexpression or altered signaling has been observed in several cancers where it may contribute to tumor growth and immune evasion. The interaction between PVR and its related protein TIGIT can affect antitumor immune responses complicating cancer progression. Additionally as its name suggests PVR binds to the poliovirus facilitating viral entry and spread during infection. This highlights the importance of PVR not only in pathogenic interactions but also in broader immune response contexts.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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제품 프로토콜

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Product promise

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