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AB258622

Human RAD1 knockout HeLa cell lysate

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RAD1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2 and 62 bp deletion in exon2.

대체 명칭 보기

RAD1, REC1, Rad1-like DNA damage checkpoint protein, hRAD1, Cell cycle checkpoint protein RAD1

3 이미지
Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)
  • Sanger seq

Unknown

Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)

Allele-2 : 1 bp insertion in exon2

Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)
  • Sanger seq

Unknown

Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)

Allele-3 : 62 bp deletion in exon2

Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)
  • Sanger seq

Unknown

Sanger Sequencing - Human RAD1 knockout HeLa cell lysate (AB258622)

Allele-1 : 2 bp deletion in exon2

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp deletion in exon2 and 62 bp deletion in exon2.

질병

Adenocarcinoma

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보완 제품

Primary Antibodies

AB168338

Anti-CLPX antibody [EP8772]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-CLPX antibody [EP8772] (AB168338)

  • 3
  • 2
Primary Antibodies

AB76148

Anti-MSI2 antibody [EP1305Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody [EP1305Y] (AB76148)

  • 5
  • 1
Primary Antibodies

AB184451

Anti-Dopamine Transporter antibody [EPR19695]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (AB184451)

  • 0
  • 0
Primary Antibodies

AB181150

Anti-G3BP antibody [EPR13986(B)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP antibody [EPR13986(B)] (AB181150)

  • 5
  • 1
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258622-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258622-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human RAD1 knockout HeLa cell lysate", "number":"AB258622-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
RAD1
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Rad1 also known as RAD1 homolog 1 from Saccharomyces cerevisiae functions mechanically as an exonuclease involved in DNA repair processes. It forms a part of the 9-1-1 checkpoint complex which acts as a sensor for DNA damage. Rad1 has a molecular mass of approximately 34 kDa and is expressed in various tissues where cell division occurs. Its primary role is in maintaining genomic stability by participating in the recognition and repair of abnormal DNA structures.
Biological function summary

Rad1 plays an essential role in the DNA damage response by being part of the 9-1-1 complex which includes Rad9 and Hus1 proteins. This complex is an important player in activating the ATR signaling pathway which helps cells respond to DNA replication stress and damage. Rad1 helps ensure proper checkpoint activation preventing cells with damaged DNA from progressing through the cell cycle which further safeguards genomic integrity.

Pathways

The protein Rad1 functions critically within the ATR-Chk1 signaling pathway a significant route for controlling cell cycle checkpoints. In this context Rad1 associates with proteins such as ATR and Chk1 facilitating the signaling necessary for cell cycle arrest under conditions of DNA stress. Rad1 also interacts with the base excision repair pathway where it works with other repair proteins to rectify oxidative DNA damage and maintain cellular viability.

Rad1 has a connection with cancer due to its involvement in DNA repair pathways. Impaired Rad1 function can lead to accumulation of DNA damage contributing to carcinogenesis. Additionally Rad1’s participation in maintaining genomic stability links it to disorders like Fanconi anemia where DNA repair defects are evident. In these contexts Rad1's interactions with proteins like BRCA1 further highlight its importance in protective cellular mechanisms.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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