REC8 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 95 bp deletion in exon6.
대체 명칭 보기
REC8, Meiotic recombination protein REC8 homolog, Cohesin Rec8p, REC8L1
- Sanger seq
Unknown
Sanger Sequencing - Human REC8 knockout A549 cell lysate (AB257633)
Homozygous : 95 bp deletion in exon6
보완 제품
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REC8 antibody [EPR16189] (AB192241)](https://content.abcam.com/products/images/rec8-antibody-epr16189-ab192241--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18847.gif)
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Sister chromatid cohesion during meiosis depends heavily on REC8 which integrates into the cohesin protein complex. This complex consists of SMC1β SMC3 and STAG3 playing an essential role in meiotic chromosome pairing and segregation. REC8 forms part of this cohesin structure specifically ensuring precise cleavage and separation of chromatids during the anaphase I stage of meiosis which contributes critical fidelity in chromosomal inheritance.
Pathways
REC8 influences the meiotic cell cycle and the associated recombination pathways. These pathways are highly regulated and critical for gametogenesis. Within the meiotic cell cycle pathway REC8 interacts closely with the protein RAD21 which holds key roles across various stages of the cell cycle. The well-orchestrated removal of cohesion by separase enzyme ensures successful homologous chromosome separation highlighting REC8's importance in these cellular processes.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com