- Sanger seq
Unknown
Sanger Sequencing - Human RPL22 knockout HEK-293T cell lysate (AB258176)
Homozygous : 2 bp deletion in exon 1
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
Zygosity
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RPL22 contributes to ribosomal biogenesis by being part of a larger ribonucleoprotein complex. It participates in the assembly of the 60S subunit of ribosomes which is essential for translation. The proper function of RPL22 ensures accurate and efficient polypeptide elongation during protein synthesis. Being a part of the ribosomal machinery RPL22 impacts cell proliferation and growth as ribosomes are critical for producing proteins needed for cell development and function.
Pathways
RPL22 plays a role in the translation machinery impacting the protein synthesis pathway. It interacts with other ribosomal proteins such as RPL5 and RPL10 which together facilitate the translation of mRNA into protein. Additionally RPL22 has relevance in the mTOR signaling pathway an important regulator of cell growth and proliferation where it affects processes by influencing ribosomal output and protein translation directly.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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