RPL36A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
대체 명칭 보기
60S ribosomal protein L44, Large ribosomal subunit protein eL42, GIG15, MIG6, RPL36A, 60S ribosomal protein L36a, RPL44, Cell migration-inducing gene 6 protein, Cell growth-inhibiting gene 15 protein
- Sanger seq
Unknown
Sanger Sequencing - Human RPL36A knockout HeLa cell lysate (AB259089)
Allele-2 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RPL36A knockout HeLa cell lysate (AB259089)
Allele-1 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RPL36A knockout HeLa cell lysate (AB259089)
Allele-3 : Insertion of the selection cassette in exon1
보완 제품
Primary Antibodies
AB219207
BOND RX™ Validated|RabMAb|Recombinant|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (AB219207)](https://content.abcam.com/products/images/pdx1-antibody-epr22002-ab219207--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81218.jpg)
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제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RPL36A is involved in the ribosome's structural integrity and function. It assumes an important role within the ribosomal large subunit by engaging in ribosome assembly and stability. As a part of the ribosomal complex RPL36A contributes to the decoding and translocation phases during protein biosynthesis. Its integration within this complex highlights its significance in cellular metabolism and growth.
Pathways
RPL36A plays an integral part in the translation pathway specifically in the process of translation initiation and elongation. It is actively involved in the assembly of the pre-initiation complex and the elongation cycle. Additionally RPL36A interacts within the mTOR signaling pathway which regulates cell growth and metabolism in response to nutrients growth factors and other stimuli. Through this pathway its relationship with proteins like S6 kinase is noteworthy as they collectively influence protein synthesis rate and cell growth.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com