JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258648

Human SAMD9L knockout A549 cell lysate

Be the first to review this product! 리뷰 제출

|

(0 출판물)

SAMD9L KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon5 and 1 bp deletion in exon5.
2 이미지
Sanger Sequencing - Human SAMD9L knockout A549 cell lysate (AB258648)
  • Sanger seq

Unknown

Sanger Sequencing - Human SAMD9L knockout A549 cell lysate (AB258648)

Allele-2 : 1 bp deletion in exon5

Sanger Sequencing - Human SAMD9L knockout A549 cell lysate (AB258648)
  • Sanger seq

Unknown

Sanger Sequencing - Human SAMD9L knockout A549 cell lysate (AB258648)

Allele-1 : 11 bp deletion in exon5

주요 정보

세포 유형

A549

Species or organism

Human

조직

Lung

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon5 and 1 bp deletion in exon5.

질병

Carcinoma

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258648-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type A549 cell lysate", "number":"AB258648-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human SAMD9L knockout A549 cell lysate", "number":"AB258648-CMP02", "productcode":"" } ] } } }

특성 및 보관 정보

유전자명
SAMD9L
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SAMD9L also known as Sterile Alpha Motif Domain-Containing Protein 9-Like plays a significant role in regulating cellular proliferation. This protein weighs approximately 181 kDa and is expressed in several tissues with higher levels observed in the bone marrow and lymphoid tissues. SAMD9L functions mechanically by modulating cellular responses to growth signals thereby influencing various cellular activities.
Biological function summary

The SAMD9L protein is involved in important cellular processes and hematopoiesis. Through its role in regulating immune responses and cell growth it interacts with other proteins to form functional complexes that facilitate homeostasis in the body. Its presence impacts how cells react to external stress and signals affecting both normal and pathological conditions.

Pathways

SAMD9L interacts with elements of the innate immune system and the cellular stress response pathways. It engages with the JAK-STAT signaling pathway affecting transcriptional activity linked to cell proliferation and survival. SAMD9L also shows interactions with proteins like STAT1 and STAT3 influencing signal transduction processes that maintain cell equilibrium under stress conditions.

Researchers have linked SAMD9L to myelodysplastic syndromes and hematopoietic malignancies. Mutations or dysregulations of SAMD9L can lead to aberrant cell growth and differentiation contributing to disease progression. It is associated with other molecules such as interferon-alpha which can modulate the inflammatory response in these conditions providing specific targets for diagnostic and therapeutic approaches.

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

제품 프로토콜

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com