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AB259135

Human SLC43A3 knockout HeLa cell lysate

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SLC43A3 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.
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Sanger Sequencing - Human SLC43A3 knockout HeLa cell lysate (AB259135)
  • Sanger seq

Unknown

Sanger Sequencing - Human SLC43A3 knockout HeLa cell lysate (AB259135)

Homozygous : 1 bp deletion in exon 3

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.

질병

Adenocarcinoma

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

제품 구성

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특성 및 보관 정보

유전자명
SLC43A3
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The SLC43A3 protein also known as ENBT3 functions as a transporter for nucleobases like adenine and hypoxanthine. It belongs to the SLC43 family specifically facilitating the movement of nucleobases across the cell membrane. This protein has an approximate molecular mass of 51 kDa and is expressed in various tissues including the liver kidney and placenta. SLC43A3 plays an important role in regulating nucleotide levels within cells and maintaining a balance necessary for many cellular processes.
Biological function summary

This protein ensures the uptake of essential nucleobases contributing to nucleotide biosynthesis and salvage pathways. SLC43A3 does not function as part of a larger protein complex. Its role in transporting nucleobases is significant for cellular RNA and DNA synthesis indicating its involvement in cellular proliferation and repair. This highlights its importance in cellular growth and energy metabolism.

Pathways

SLC43A3 actively contributes to the purine salvage pathway critical for recycling nucleobases back into nucleotides. The purine salvage pathway is integral for conserving energy in cells as it allows the reuse of preformed bases. SLC43A3 is closely related to proteins like HPRT1 and APRT which also participate in this pathway. These interactions enable the efficient functioning of nucleotide metabolism and synthesis within various cell types.

Alterations in SLC43A3 expression or function may contribute to conditions such as cancer and certain metabolic disorders. Cancer in particular often involves enhanced nucleotide synthesis and salvage pathways to support rapid cell division. SLC43A3 by adjusting nucleotide availability can influence the progression of such diseases. Its interaction with other nucleotide-related proteins can also complicate metabolic diseases. Recognizing these links is helpful for understanding the molecular basis of these diseases and potentially targeting SLC43A3 for therapeutic interventions.

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

제품 프로토콜

Product promise

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