Human SNAP25 knockout SH-SY5Y cell lysate

• WB

Lab

Western blot - Human SNAP25 knockout SH-SY5Y cell lysate (AB280100)

Lane 1 : Wild-type SH-SY5Y cell lysate 20 μg
Lane 2 : SNAP25 knockout SH-SY5Y cell lysate 20 μg
Lane 3 : Neuro-2a cell lysate 20 μg
Lane 4 : K562 cell lysate 20 μg
False colour image of Western blot : Anti-SNAP25 antibody [EPR3275] staining at 1/1000 dilution, shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab109105 was shown to bind specifically to SNAP25. A band was observed at 27 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in SNAP25 knockout cell line ab280041 (knockout cell lysate ab280100). To generate this image, wild-type and SNAP25 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-SNAP25 antibody [EPR3275] (<a href='/ko/products/primary-antibodies/snap25-antibody-epr3275-ab109105'>ab109105</a>) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 2:

SNAP25 knockout SH-SY5Y cell lysate at 20 µg

Lane 2:

Western blot - Human SNAP25 knockout SH-SY5Y cell line (<a href='/ko/products/cell-lines/human-snap25-knockout-sh-sy5y-cell-line-ab280041'>ab280041</a>)

Lane 3:

Neuro-2a cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

• WB

Lab

Western blot - Human SNAP25 knockout SH-SY5Y cell lysate (AB280100)

Lane 1 : Wild-type SH-SY5Y cell lysate 20 μg
Lane 2 : SNAP25 knockout SH-SY5Y cell lysate 20 μg
Lane 3 : Neuro-2a cell lysate 20 μg
Lane 4 : K562 cell lysate 20 μg
False colour image of Western blot : Anti-SNAP25 antibody [EP3274] staining at 1/1000 dilution, shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab108990 was shown to bind specifically to SNAP25. A band was observed at 27 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in SNAP25 knockout cell line ab280041 (knockout cell lysate ab280100). To generate this image, wild-type and SNAP25 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-SNAP25 antibody [EP3274] (<a href='/ko/products/primary-antibodies/snap25-antibody-ep3274-ab108990'>ab108990</a>) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 2:

SNAP25 knockout SH-SY5Y cell lysate at 20 µg

Lane 2:

Western blot - Human SNAP25 knockout SH-SY5Y cell line (<a href='/ko/products/cell-lines/human-snap25-knockout-sh-sy5y-cell-line-ab280041'>ab280041</a>)

Lane 3:

Neuro-2a cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

• Sanger seq

Supplier Data

Sanger Sequencing - Human SNAP25 knockout SH-SY5Y cell lysate (AB280100)

Human SNAP25 KO in SH-SY5Y Cells with 34 bp deletion in exon 1, CCDS13110.1.