- Sanger seq
Unknown
Sanger Sequencing - Human SSR4 knockout HEK-293T cell lysate (AB259157)
Allele-1 : 10 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SSR4 knockout HEK-293T cell lysate (AB259157)
Allele-2 : 8 bp deletion in exon 1
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SSR4 functions as part of the signal sequence receptor (SSR) complex. This complex is essential for the targeting and translocation of newly synthesized proteins into the endoplasmic reticulum lumen. SSR4 interacts directly with signal sequences of nascent polypeptides to ensure their correct orientation and localization during protein synthesis. Proper functioning of SSR4 is necessary for the homeostasis of the cellular secretory pathway.
Pathways
SSR4 is involved in the protein translocation pathway which is essential for protein sorting and trafficking. This pathway intersects with the unfolded protein response (UPR) pathway which maintains protein homeostasis under stress conditions. In these pathways SSR4 collaborates with proteins like Sec61 to mediate translocation and assist in protein folding. Disruptions in these pathways can lead to cellular dysfunction and stress-related responses.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com