- Sanger seq
Unknown
Sanger Sequencing - Human TAF11 knockout HeLa cell lysate (AB259172)
Allele-2 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human TAF11 knockout HeLa cell lysate (AB259172)
Allele-1 : 5 bp deletion in exon1
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TAF11 functions as a core component of the TFIID complex which consists of the TATA-binding protein (TBP) and multiple TBP-associated factors (TAFs). The complex plays an important role in the regulation of gene expression by assisting in the formation of the transcription preinitiation complex at gene promoters. By interacting with other subunits within the TFIID complex TAF11 helps in the precise transcription of genes supporting cellular processes.
Pathways
TAF11 plays a central role in the transcription regulation pathway and is also involved in the RNA polymerase II transcription initiation pathway. It interacts with multiple factors in these pathways including TAF6 and TAF9 which are other TAF proteins that collaborate in stabilizing the transcription preinitiation complex. These interactions support its involvement in the regulation of both basal and activated transcription influencing gene expression patterns.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com