Human TLE1 knockout HEK-293T cell lysate

• WB

Unknown

Western blot - Human TLE1 knockout HEK-293T cell lysate (AB257240)

Lane 1 : Wild-type HEK293T cell lysate (20 μg)

Lane 2 : TLE1 knockout HEK293T cell lysate (20 μg)

Lane 3 : HepG2 cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab183742 observed at 83 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab183742 Anti-TLE 1 antibody [EPR9386(2)] was shown to specifically react with TLE 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265059 (knockout cell lysate ab257240) was used. Wild-type and TLE 1 knockout samples were subjected to SDS-PAGE. ab183742 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TLE 1 antibody [EPR9386(2)] (<a href='/ko/products/primary-antibodies/tle-1-antibody-epr93862-ab183742'>ab183742</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TLE1 knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-tle1-knockout-hek-293t-cell-line-ab265059'>ab265059</a>) at 20 µg

Lane 2:

TLE1 knockout HEK293T cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

• WB

Unknown

Western blot - Human TLE1 knockout HEK-293T cell lysate (AB257240)

Lane 1 : Wild-type HEK293T cell lysate (20 μg)

Lane 2 : TLE1 knockout HEK293T cell lysate (20 μg)

Lane 3 : HepG2 cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab131648 observed at 83 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab131648 Anti-TLE 1 antibody [OTI1F5] was shown to specifically react with TLE 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265059 (knockout cell lysate ab257240) was used. Wild-type and TLE 1 knockout samples were subjected to SDS-PAGE. ab131648 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Anti-TLE 1 antibody [OTI1F5] (<a href='/ko/products/unavailable/tle-1-antibody-oti1f5-ab131648'>ab131648</a>) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TLE1 knockout HEK293T cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

false

• Sanger seq

Unknown

Sanger Sequencing - Human TLE1 knockout HEK-293T cell lysate (AB257240)

Allele-1 : 13 bp deletion in exon 12

• Sanger seq

Unknown

Sanger Sequencing - Human TLE1 knockout HEK-293T cell lysate (AB257240)

Allele-2 : Insertion of the selection cassette in exon 12