AB258233

Human TMED10 (TMP21) knockout HEK-293T cell lysate

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TMED10 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.

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Lab

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : TMED10 knockout HEK293T cell lysate (20 ug)
Lane 3 : U-87 MG cell lysate (20 ug)
Lane 4 : Raji cell lysate (20 ug)

ab133771 was shown to specifically react with TMP21 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266227 (knockout cell lysate ab258233) was used. Wild-type and TMP21 knockout samples were subjected to SDS-PAGE. ab133771 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TMP21 antibody [EPR9037(B)] (<a href='/ko/products/primary-antibodies/tmp21-antibody-epr9037b-ab133771'>ab133771</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TMED10 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-tmed10-tmp21-knockout-hek-293t-cell-line-ab266227'>ab266227</a>)

Lane 3:

U-87 MG cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 25 kDa,27 kDa,37 kDa,46 kDa

Observed band size: 19 kDa,27 kDa

false

Sanger Sequencing - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Sanger seq

Unknown

Sanger Sequencing - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Homozygous : 2 bp insertion in exon1

Lab

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : TMED10 knockout HEK293T cell lysate (20 ug)
Lane 3 : U-87 MG cell lysate (20 ug)
Lane 4 : Raji cell lysate (20 ug)

ab134948 was shown to specifically react with TMP21 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266227 (knockout cell lysate ab258233) was used. Wild-type and TMP21 knockout samples were subjected to SDS-PAGE. ab134948 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TMP21 antibody [EPR9036(B)] (<a href='/ko/products/primary-antibodies/tmp21-antibody-epr9036b-ab134948'>ab134948</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (ab258233) at 20 µg

Lane 3:

U-87 MG cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 25 kDa,9 kDa

Observed band size: 13 kDa,19 kDa

false

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258233-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB258233-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human TMED10 knockout HEK293T cell lysate", "number":"AB258233-CMP01", "productcode":"" } ] } } }

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특성 및 보관 정보

유전자명

TMED10

유전자 편집 종류

Knockout

유전자 편집 방식

CRISPR technology

Knockout 검증

Sanger Sequencing, Western blot

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TMP21 also known as transmembrane protein 21 or by its alternate name p23 is a protein that plays a significant role in cellular functions. It has an approximate molecular mass of 21 kDa. TMP21 is mainly expressed in the brain but is also found in a variety of other tissues. Mechanically TMP21 functions as a part of the secretory pathway within the cell contributing to the processing and maturation of nascent proteins. It is known to regulate the trafficking of proteins across cellular compartments which is an important aspect of maintaining cellular homeostasis.

Biological function summary

TMP21 affects the modulation of the gamma-secretase complex which is essential in the generation of amyloid precursor protein (APP) and its subsequent processing. This protein acts as a negative regulator of gamma-secretase activity leading to alterations in APP cleavage. By being part of this complex TMP21 exerts influence over processes such as signal transduction and protein metabolism. Its expression levels and activity can impact neurophysiological functions due to its presence in nervous tissues.

Pathways

TMP21 integrates into the amyloidogenic pathway influencing amyloid-beta peptide production. This pathway is associated with the APP processing where TMP21 regulates the cleavage by controlling gamma-secretase activity. Another pathway involving TMP21 is the protein transport pathway facilitating the orderly movement of proteins through the early secretory pathway. Within these frameworks TMP21 interacts with proteins like presenilin which is a significant component of the gamma-secretase complex thereby affecting its overall functionality.

TMP21 has strong links to Alzheimer's disease and cancer. In Alzheimer's disease altered TMP21 function can lead to improper processing of APP resulting in increased amyloid-beta production and plaque formation. This relationship highlights TMP21's connection with presenilin which is important in amyloidogenic processes and influences disease progression. Additionally fluctuations in TMP21 expression and activity have been observed in certain cancers suggesting its role in aberrant cellular growth and migration due to its involvement in protein trafficking pathways. Understanding TMP21's interactions in these diseases can provide insights into potential therapeutic targets.

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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human TMED10 (TMP21) knockout HEK-293T cell lysate

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Sanger Sequencing - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

주요 정보

세포 유형

Species or organism

조직

Knockout 검증

Mutation 정보

보완 제품

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

유전자명

유전자 편집 종류

유전자 편집 방식

Knockout 검증

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

품질 관리

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

제품 프로토콜

Product promise