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AB263392

Human TMEM59 knockout HEK-293T cell lysate

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TMEM59 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.

대체 명칭 보기

Transmembrane protein 59, Liver membrane-bound protein, UNQ169/PRO195, TMEM59, HSPC001, C1orf8

1 이미지
Sanger Sequencing - Human TMEM59 knockout HEK-293T cell lysate (AB263392)
  • Sanger seq

Unknown

Sanger Sequencing - Human TMEM59 knockout HEK-293T cell lysate (AB263392)

Homozygous : Insertion of the selection cassette in exon1

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

제품 구성

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특성 및 보관 정보

유전자명
TMEM59
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TMEM59 also known as Transmembrane Protein 59 plays a role in protein glycosylation and modulation of the cell surface expression of certain proteins. It has a molecular mass of approximately 39 kDa. TMEM59 is expressed in a variety of tissues including the brain lung and kidney where it localizes to the endoplasmic reticulum and Golgi apparatus. The protein's structure suggests membrane spanning properties that contribute to its function in cell regulation.
Biological function summary

TMEM59 contributes to proper neuronal function by modulating the cell surface expression of proteins involved in neuron-neuron communications. This regulation has implications for membrane protein function and synaptic stability. TMEM59 participates in certain bionetworks interacting with protein complexes that influence cell signaling pathways. Such interactions play significant roles in controlling protein trafficking and localization within cells impacting the cellular response to environmental signals.

Pathways

TMEM59 influences processes like the Wnt signaling pathway affecting cell fate decisions and embryonic development. It interacts with proteins such as β-catenin and affects their stability and function. TMEM59 also participates in the autophagy-dependent pathways modulating cellular mechanisms involved in the degradation of damaged proteins and organelles therefore maintaining cellular homeostasis.

TMEM59 has links to neurodegenerative diseases including Alzheimer's disease and schizophrenia. TMEM59's regulation of proteins like APP (Amyloid precursor protein) is significant. Disruptions in its function may contribute to aberrant protein aggregation commonly seen in Alzheimer's disease pathology. Additionally disruptions in TMEM59 expression or function could affect cognitive processes and synaptic connections related to schizophrenia as it influences the trafficking of receptor proteins important for synaptic signaling.

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

제품 프로토콜

Product promise

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