Human TMSB10 (Thymosin beta 10) knockout HEK-293T cell lysate
Be the first to review this product! 리뷰 제출
|
(0 출판물)
TMSB10 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2 and 5 bp deletion in exon2.
대체 명칭 보기
TMSB10, Thymosin beta-10, PTMB10, THYB10
- Sanger seq
Unknown
Sanger Sequencing - Human TMSB10 (Thymosin beta 10) knockout HEK-293T cell lysate (AB259193)
Allele-2 : 4 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TMSB10 (Thymosin beta 10) knockout HEK-293T cell lysate (AB259193)
Allele-1 : 5 bp deletion in exon2
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TMSB10 significantly influences cell movement and proliferation. It serves as an actin-sequestering protein and is not generally part of a larger protein complex. TMSB10 promotes cell motility and plays an essential part in processes like wound healing and angiogenesis. Through these actions it helps in tissue regeneration and repair influencing the cell's structural organization and signaling.
Pathways
TMSB10 is involved in the actin cytoskeleton pathway and cellular signaling pathways that govern movement and shape changes in cells. Its interaction with actin places it in relation to actin-related protein (ARP) complexes such as Arp2/3 which facilitate actin filament branching. In addition TMSB10's regulation of actin dynamics links it to pathways involving proteins like cofilin which modulates actin filament turnover.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com