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AB257067

Human TSPO (PBR) knockout HCT116 cell lysate

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TSPO KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon2.

대체 명칭 보기

BZRP, PKBS, TSPO, Peripheral-type benzodiazepine receptor, MBR, Mitochondrial benzodiazepine receptor, Translocator protein, PBR

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Sanger Sequencing - Human TSPO (PBR) knockout HCT116 cell lysate (AB257067)
  • Sanger seq

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Sanger Sequencing - Human TSPO (PBR) knockout HCT116 cell lysate (AB257067)

Homozygous : Insertion of the selection cassette in exon2

Western blot - Human TSPO (PBR) knockout HCT116 cell lysate (AB257067)
  • WB

Lab

Western blot - Human TSPO (PBR) knockout HCT116 cell lysate (AB257067)

Lane 1 : Wild-type HCT116 cell lysate (20µg)

Lane 2 : TSPO knockout HCT116 cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab109497 observed at 17 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109497 Anti-PBR antibody [EPR5384] was shown to specifically react with PBR in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266878 (knockout cell lysate ab257067) was used. Wild-type and PBR knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109497 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PBR antibody [EPR5384] (<a href='/ko/products/primary-antibodies/pbr-antibody-epr5384-ab109497'>ab109497</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human TSPO (PBR) knockout HCT116 cell lysate (ab257067) at 20 µg

Predicted band size: 19 kDa

Observed band size: 17 kDa

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주요 정보

세포 유형

HCT116

Species or organism

Human

조직

Colon

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon2.

질병

Carcinoma

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보완 제품

Primary Antibodies

AB109497

Anti-PBR antibody [EPR5384]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBR antibody [EPR5384] (AB109497)

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Biochemicals

AB120378

PK11195, benzodiazepine receptor antagonist

Chemical Structure - PK11195, benzodiazepine receptor antagonist (AB120378)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257067-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human TSPO knockout HCT116 cell lysate", "number":"AB257067-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HCT116 cell lysate", "number":"AB257067-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
TSPO
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing, Western blot
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PBR also known as the Peripheral Benzodiazepine Receptor is a protein predominantly found in the outer mitochondrial membrane. It has alternate names like Translocator Protein (TSPO) and the mass of this protein is approximately 18 kDa. PBR is expressed in various tissues but shows high levels in steroidogenic tissues like adrenal glands as well as in the brain heart liver and kidneys. The abundant presence in these tissues highlights its importance in a variety of physiological functions.
Biological function summary

PBR interacts with cholesterol for the synthesis of steroid hormones making it important for steroidogenesis. PBR is a part of the Mitochondrial Permeability Transition Pore complex (MPTP) involved in regulating the transport of molecules across the mitochondrial membrane. Through its association with the MPTP PBR plays a significant role in mitochondrial functions such as apoptosis and energy metabolism. The interaction with other molecules also includes the binding with benzodiazepines impacting processes like immune response and cell proliferation.

Pathways

PBR plays a role in the steroid biosynthesis and apoptosis pathways. It interfaces with the StAR (Steroidogenic Acute Regulatory) protein to facilitate cholesterol transport into mitochondria the initial step in steroid hormone production. PBR is also involved in pathways that regulate apoptosis and mitochondrial function linking it to different cellular processes through interactions with proteins like VDAC (Voltage-Dependent Anion Channel).

PBR has relevance to conditions such as neurodegenerative diseases and cancer. PBR expression changes in disorders like Alzheimer's disease where it might reflect mitochondrial dysfunctions. It has also been associated with certain cancers where aberrant PBR activity might contribute to altered cell proliferation and apoptosis. Proteins like caspases are involved in the apoptotic pathways connected with PBR highlighting its involvement in disease processes.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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제품 프로토콜

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Product promise

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