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AB263773

Human UCHL1 (PGP9.5) knockout HEK-293T cell lysate

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UCHL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 45 bp deletion in exon 1.
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Western blot - Human UCHL1 (PGP9.5) knockout HEK-293T cell lysate (AB263773)
  • WB

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Western blot - Human UCHL1 (PGP9.5) knockout HEK-293T cell lysate (AB263773)

Lane 1 : Wild-type Hap1 cell lysate (20 μg)

Lane 2 : UCHL1 knockout Hap1 cell lysate (20 μg)

Lane 3 : SH-SY5Y cell lysate (20 μg)

Lane 4 : Wild-type HEK-293T cell lysate (20 μg)

Lane 5 : UCHL1 knockout HEK-293T cell lysate (20 μg)

Lanes 1 - 5 : Merged signal (red and green). Green - ab108986 observed at 25 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab108986 was shown to react with PGP9.5 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255443 (knockout cell lysate ab263773) was used. Wild-type and PGP9.5 knockout samples were subjected to SDS-PAGE. ab108986 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (<a href='/ko/products/primary-antibodies/pgp95-antibody-epr4118-neuronal-marker-ab108986'>ab108986</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

UCHL1 knockout HAP1 cell lysate at 20 µg

Lane 2:

Western blot - Human UCHL1 (PGP9.5) knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-uchl1-pgp95-knockout-hek-293t-cell-line-ab255443'>ab255443</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Wild-type HEK-293T cell lysate at 20 µg

Lane 5:

UCHL1 knockout HEK-293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa,37 kDa

false

Sanger Sequencing - Human UCHL1 (PGP9.5) knockout HEK-293T cell lysate (AB263773)
  • Sanger seq

Unknown

Sanger Sequencing - Human UCHL1 (PGP9.5) knockout HEK-293T cell lysate (AB263773)

Homozygous : 45 bp deletion in exon 1

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 45 bp deletion in exon 1.

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보완 제품

Assay Kits

AB244190

Human/Mouse PGP9.5 Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human/Mouse PGP9.5 Antibody Pair - BSA and Azide free (AB244190)

  • 0
  • 0
Primary Antibodies

AB108986

Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-PGP9.5 antibody [EPR4118] - Neuronal Marker (AB108986)

  • 5
  • 12
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0
Proteins & Peptides

AB280753

Recombinant Human PD1 protein

Mass Spectrometry - Recombinant Human PD1 protein (AB280753)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263773-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human UCHL1 knockout HEK293T cell lysate", "number":"AB263773-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB263773-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
UCHL1
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing, Western blot
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.
Biological function summary

PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.

Pathways

PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.

Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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