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AB259232

Human USP40 knockout HeLa cell lysate

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USP40 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

대체 명칭 보기

Ubiquitin thioesterase 40, Ubiquitin-specific-processing protease 40, Ubiquitin carboxyl-terminal hydrolase 40, Deubiquitinating enzyme 40, USP40

1 이미지
Sanger Sequencing - Human USP40 knockout HeLa cell lysate (AB259232)
  • Sanger seq

Unknown

Sanger Sequencing - Human USP40 knockout HeLa cell lysate (AB259232)

Homozygous : 1 bp deletion in exon 1

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

질병

Adenocarcinoma

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

제품 구성

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특성 및 보관 정보

유전자명
USP40
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

USP40 also known as Ubiquitin Specific Peptidase 40 functions as a deubiquitinating enzyme. It belongs to a family of enzymes that remove ubiquitin from proteins. USP40 has a molecular mass of approximately 140 kDa. The expression of USP40 occurs mainly in tissues like the brain and testis. It lacks deubiquitinase activity on some substrates indicating specificity in its action compared to other ubiquitin proteases.
Biological function summary

The role of USP40 in cellular processes centers on regulating protein degradation. This regulation typically affects the stability and function of target proteins. It is not known to form a complex with other proteins but its action can still influence cellular homeostasis and stress responses indirectly. If not correctly regulated protein degradation can impact many cellular processes leading to varied biological outcomes.

Pathways

Several components of the ubiquitin-proteasome system involve USP40. This system plays an important role in maintaining protein quality control within cells. It interacts with proteins such as ubiquitin C-terminal hydrolase L1 (UCHL1) to modulate protein turnover and stability. Another pathway significantly influenced by USP40 is the autophagy-lysosome pathway where it impacts the recycling and degradation of cellular components.

Irregularities in USP40 have associations with neurodegenerative conditions like Parkinson's disease. In these contexts the dysfunction of protein degradation pathways contributes to the accumulation of misfolded proteins. USP40's association with Alzheimer's disease arises from its interaction with proteins involved in synaptic function and degradation processes like presenilin-1 (PSEN1). Enhancing the understanding of USP40 functions could provide insights into therapeutic strategies for these diseases.

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

제품 프로토콜

Product promise

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