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AB258762

Human WDR45 knockout HeLa cell lysate

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WDR45 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon3 and 1 bp insertion in exon3.
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Sanger Sequencing - Human WDR45 knockout HeLa cell lysate (AB258762)
  • Sanger seq

Unknown

Sanger Sequencing - Human WDR45 knockout HeLa cell lysate (AB258762)

Allele-1 : 11 bp deletion in exon3

Sanger Sequencing - Human WDR45 knockout HeLa cell lysate (AB258762)
  • Sanger seq

Unknown

Sanger Sequencing - Human WDR45 knockout HeLa cell lysate (AB258762)

Allele-2 : 1 bp insertion in exon3

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon3 and 1 bp insertion in exon3.

질병

Adenocarcinoma

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

제품 구성

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특성 및 보관 정보

유전자명
WDR45
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The WDR45 protein also known as WD repeat domain phosphoinositide-interacting protein 4 (WIPI4) plays a necessary role in the autophagic process. It is a protein of approximately 42 kDa and consists of seven WD40 repeats which facilitate protein-protein interactions. The protein is broadly expressed in various tissues particularly in the brain. These interactions are critical in autophagy a process for degrading and recycling cellular components.
Biological function summary

WDR45 contributes significantly to the regulation of autophagy. It acts as part of a multi-protein complex referred to as the autophagy-related 2 (ATG) complex. This complex is involved in autophagosome formation which is an important component in cellular degradation pathways. WDR45 binds phosphoinositides which are phospholipids integral to vesicle trafficking and membrane dynamics during autophagy.

Pathways

The influence of WDR45 is evident in the PI3K/Akt signaling and AMPK signaling pathways. These pathways regulate cellular energy homeostasis and the response to metabolic stress. PI3K/Akt and AMPK modulate the activity of the mTOR pathway which is known to suppress autophagy. WDR45 interacts with other autophagy-related proteins such as LC3 and ULK1 facilitating the initiation of the autophagic process.

The connection to WDR45 is pronounced in neurodegenerative diseases like beta-propeller protein-associated neurodegeneration (BPAN) and Parkinson's disease. Mutations in WDR45 lead to defective autophagy resulting in the accumulation of damaged organelles and proteins in neurons. It is linked to proteins involved in these disorders such as PINK1 and Parkin further highlighting its potential role in disease pathology through impaired mitochondrial quality control and neural damage.

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

제품 프로토콜

Product promise

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