LNCaP nuclear extract lysate

2 Images

• WB

Unknown

Western blot - LNCaP nuclear extract lysate (AB14857)

Human Keratin, type II cytoskeletal 8 contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern observed.

All lanes:

Western blot - Anti-Cytokeratin 8 antibody (ab87883) at 1 µg/mL

Lane 1:

PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate at 10 µg

Lane 2:

Western blot - LNCaP nuclear extract lysate (ab14857) at 10 µg

Lane 3:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 53 kDa

Observed band size: 50 kDa,53 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - LNCaP nuclear extract lysate (AB14857)

CD10 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-CD10 antibody (ab73409) at 1 µg/mL

Lane 1:

Western blot - LNCaP nuclear extract lysate (ab14857) at 10 µg

Lane 2:

Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Human kidney tissue lysate - total protein (ab30203) at 10 µg

Lane 5:

Human placenta tissue lysate - total protein (ab29745) at 10 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

Predicted band size: 85 kDa

Observed band size: 100 kDa,38 kDa

true

Exposure time: 2min