Munc18-1 overexpression 293T lysate (whole cell)

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Munc18-1 also known as Syntaxin-binding protein 1 is an important protein involved in the regulation of neurotransmitter release at synapses. It has a molecular mass of approximately 67 kDa. This protein is highly expressed in the brain where it plays a significant role in synaptic vesicle exocytosis. Munc18-1 interacts with components of the SNARE complex facilitating the docking and fusion of synaptic vesicles with the presynaptic membrane.

Biological function summary

This protein is important for the proper release of neurotransmitters into the synaptic cleft. Munc18-1 functions as a part of the neuronal exocytosis complex where it regulates the interaction with syntaxin-1 a core SNARE protein. This protein-protein interaction stabilizes an intermediate state necessary for vesicle priming and subsequent fusion ensuring efficient synaptic transmission. By controlling vesicle movement Munc18-1 influences communication between neurons.

Pathways

Munc18-1 integrates into the neurotransmitter release pathway which is central to synaptic function. It closely associates with the SNARE complex involving proteins such as SNAP-25 and synaptobrevin. Another important pathway is the synaptic vesicle cycle where Munc18-1 plays a role in vesicle docking and priming stages. Through these interactions it ensures that synaptic vesicles are ready and able to release their contents upon stimulation.

Alterations in Munc18-1 expression or function connect to neurological conditions like epilepsy and intellectual disability. In epilepsy dysregulation of neurotransmitter release due to modified Munc18-1 function disrupts synaptic communication leading to seizure activity. In the context of these disorders Munc18-1 interacts functionally with proteins such as VAMP2 contributing to the pathological state when disrupted. These interactions highlight the importance of Munc18-1 in maintaining normal neurological function.