JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB30124

Raji whole cell lysate

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Raji whole cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
4 이미지
Western blot - Raji whole cell lysate (AB30124)
  • WB

Project

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-SOCS2 antibody (<a href='/ko/products/primary-antibodies/socs2-antibody-ab66733'>ab66733</a>) at 1 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Western blot - SW480 whole cell lysate (<a href='/ko/products/cell-lysates/sw480-whole-cell-lysate-ab3957'>ab3957</a>) at 10 µg

Lane 4:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 5:

Western blot - Mouse thymus tissue lysate - total protein (<a href='/ko/products/tissue-lysates/mouse-thymus-tissue-lysate-total-protein-ab29285'>ab29285</a>) at 10 µg

Lane 6:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Western blot - Raji whole cell lysate (AB30124)
  • WB

Project

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-BRD1/BRL antibody (<a href='/ko/products/primary-antibodies/brd1-brl-antibody-ab71877'>ab71877</a>) at 1 µg/mL

Lane 1:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

Lane 2:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Observed band size: 150 kDa

false

Western blot - Raji whole cell lysate (AB30124)
  • WB

Project

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-HYAL2 antibody (<a href='/ko/products/primary-antibodies/hyal2-antibody-ab68608'>ab68608</a>) at 1 µg/mL

Lane 1:

Liver (Rat) Tissue Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Human thymus tissue lysate - total protein (<a href='/ko/products/unavailable/human-thymus-tissue-lysate-total-protein-ab30146'>ab30146</a>) at 10 µg

Lane 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 8:

Western blot - SW480 whole cell lysate (<a href='/ko/products/cell-lysates/sw480-whole-cell-lysate-ab3957'>ab3957</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 54 kDa

Observed band size: 40 kDa,50 kDa,54 kDa,58 kDa

true

Western blot - Raji whole cell lysate (AB30124)
  • WB

Project

Western blot - Raji whole cell lysate (AB30124)

All lanes:

Western blot - Anti-ATG3 antibody (<a href='/ko/products/primary-antibodies/atg3-antibody-ab66287'>ab66287</a>) at 1 µg/mL

Lane 1:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - Raji whole cell lysate (ab30124) at 10 µg

Lane 3:

JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa,72 kDa

false

주요 정보

세포 유형

Raji

Species or organism

Human

조직

Lymphatic

제형

Liquid

form

질병

Lymphoma

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Samples need to boiled for 5 minutes and put on ice before use.</p>" } } }

제품 세부 정보

Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

특성 및 보관 정보

배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Allow to warm to room temp and agitate gently before aliquoting

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Suspension

Gender

Male

제품 프로토콜

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com