JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB156907

ChIP Kit Magnetic - One Step

Be the first to review this product! 리뷰 제출

|

(33 제품이 사용된 논문 )

ChIP Kit Magnetic - One Step (ab156907) contains reagents required for chromatin immunoprecipitation (ChIP) directly from chromatin extracts isolated from mammalian cells or tissues (fresh and frozen).

- Includes a positive control antibody (RNA polymerase II), positive control (GAPDH primers) and negative control (Non-immune IgG)
- Compatible with various downstream applications including PCR (ChIP-PCR), microarrays (ChIP-chip), and sequencing (ChIP-seq)
-Enrichment ratio of positive to negative control >200, and an extremely low number of cells required (as low as 5,000 cells per ChIP reaction)
- Proven performance: cited in over 30 publications
1 이미지
ChIP - ChIP Kit Magnetic - One Step (AB156907)
  • ChIP

Supplier Data

ChIP - ChIP Kit Magnetic - One Step (AB156907)

Typical Results.

The data shows the analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit Magnetic - One Step (ab156907) with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.

주요 정보

분석 소요 시간

5h

style
left-column

보완 제품

Sample Prep & Detection Kits

AB117152

Chromatin Extraction Kit

Functional Studies - Chromatin Extraction Kit (AB117152)

  • 0
  • 0
Sample Prep & Detection Kits

AB185913

High-Sensitivity ChIP Kit

ChIP - High-Sensitivity ChIP Kit (AB185913)

  • 0
  • 0
Sample Prep & Detection Kits

AB500

ChIP Kit

ChIP - ChIP Kit (AB500)

  • 4
  • 4
Sample Prep & Detection Kits

AB117138

ChIP Kit - One Step

ChIP - ChIP Kit - One Step (AB117138)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.</p>" } } }
style
left-column

제품 세부 정보

Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

Primers

The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.

ChIP assay products and guides

Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.

style
left-column

제품 구성

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab156907-96Test", "assetComponentDetails": [ { "size":"1 x 450 µL", "name":"Magnetic Beads", "number":"AB156907-CMP11" }, { "size":"1 x 24 Unit", "name":"8-Well Strip Caps", "number":"AB156907-CMP09" }, { "size":"1 x 2 Unit", "name":"96-Well PCR Plate", "number":"AB156907-CMP10" }, { "size":"1 x 16 µL", "name":"GAPDH Primer - Reverse (20 µM)", "number":"AB156907-CMP07" }, { "size":"1 x 28 mL", "name":"DNA Release Buffer", "number":"AB156907-CMP04" }, { "size":"1 x 12 mL", "name":"ChIP Buffer", "number":"AB156907-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB156907-CMP01" }, { "size":"1 x 16 µL", "name":"GAPDH Primer - Forward (20 µM)", "number":"AB156907-CMP05" }, { "size":"1 x 110 µL", "name":"Proteinase K (10 mg/mL)", "number":"AB156907-CMP08" }, { "size":"1 x 20 µL", "name":"Non-Immune IgG (1 mg/mL)", "number":"AB156907-CMP06" }, { "size":"1 x 16 µL", "name":"Anti-RNA Polymerase II (1 mg/mL)", "number":"AB156907-CMP02" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab156907-48Test", "assetComponentDetails": [ { "size":"1 x 220 µL", "name":"Magnetic Beads", "number":"AB156907-CMP11", "productcode":"" }, { "size":"1 x 14 mL", "name":"DNA Release Buffer", "number":"AB156907-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"10X Wash Buffer", "number":"AB156907-CMP01", "productcode":"" }, { "size":"1 x 2 Unit", "name":"96-Well PCR Plate", "number":"AB156907-CMP10", "productcode":"" }, { "size":"1 x 8 µL", "name":"GAPDH Primer - Reverse (20 µM)", "number":"AB156907-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"ChIP Buffer", "number":"AB156907-CMP03", "productcode":"" }, { "size":"1 x 8 µL", "name":"GAPDH Primer - Forward (20 µM)", "number":"AB156907-CMP05", "productcode":"" }, { "size":"1 x 12 Unit", "name":"8-Well Strip Caps", "number":"AB156907-CMP09", "productcode":"" }, { "size":"1 x 10 µL", "name":"Non-Immune IgG (1 mg/mL)", "number":"AB156907-CMP06", "productcode":"" }, { "size":"1 x 55 µL", "name":"Proteinase K (10 mg/mL)", "number":"AB156907-CMP08", "productcode":"" }, { "size":"1 x 8 µL", "name":"Anti-RNA Polymerase II (1 mg/mL)", "number":"AB156907-CMP02", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

style
left-column

제품이 사용된 논문 (33)

Recent publications for all applications. Explore the 전체 목록and refine your search

Cell reports. Medicine 6:102000 PubMed40056904

2025

LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yang,Xinyao Qiu,Yingcheng Yang,Jing Wu,Shan Wang,Bo Zheng,Jianmin Wu,Tao Zhou,Yangqianwen Zhang,Mixue Bai,Shuowu Liu,Zihan Zhao,Yani Zhang,Yixian Wang,Jinxia Bao,Mengye Wu,Dongdong Xue,Meiyu Bao,Ji Hu,Siyun Shen,Hongyang Wang,Lei Chen

Epigenetics & chromatin 17:38 PubMed39702290

2024

Dissecting the Kaiso binding profile in clear renal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey Starshin,Pavel Abramov,Yaroslava Lobanova,Fedor Sharko,Galina Filonova,Dmitry Kaluzhny,Daria Kaplun,Igor Deyev,Alexander Mazur,Egor Prokhortchou,Svetlana Zhenilo

Advanced biology 9:e2400413 PubMed39601498

2024

CBX3 Downregulates HLTF to Activate PI3K/AKT Signaling Promoting Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Min Xie,Huaiyuan Liang,Yuxuan Mao,Yuping Yao,Bingzhang Tian

iScience 27:110864 PubMed39398252

2024

RRM2 promotes liver metastasis of pancreatic cancer by stabilizing YBX1 and activating the TGF-beta pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouyuan Du,Qun Zhang,Xingxing Xiang,Wei Li,Qinglin Yang,Haixin Yu,Tao Liu

International journal of biological sciences 20:4146-4161 PubMed39247829

2024

N6-methyladenosine modification of LATS2 promotes hepatoblastoma progression by inhibiting ferroptosis through the YAP1/ATF4/PSAT1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Zhu,Yi Xie,Zhixuan Bian,Ji Ma,Ni Zhen,Tianshu Chen,Jiabei Zhu,Siwei Mao,Xiaochen Tang,Li Liu,Song Gu,Miao Ding,Qiuhui Pan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401142 PubMed39073752

2024

HDAC8 Enhances the Function of HIF-2α by Deacetylating ETS1 to Decrease the Sensitivity of TKIs in ccRCC.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Qian,Wei Li,Shangqing Ren,Weilin Peng,Bei Qing,Xinlin Liu,Xiong Wei,Liang Zhu,Yapeng Wang,Xin Jin

Journal of cellular physiology 239:e31346 PubMed38946152

2024

P7C3 ameliorates barium chloride-induced skeletal muscle injury activating transcriptomic and epigenetic modulation of myogenic regulatory factors.

Applications

Unspecified application

Species

Unspecified reactive species

Joung W Kim,Ravikumar Manickam,Puja Sinha,Wanling Xuan,Jian Huang,Kamal Awad,Marco Brotto,Srinivas M Tipparaju

Cell death and differentiation 31:592-604 PubMed38514847

2024

Retinoblastoma-associated protein is important for TRIM24-mediated activation of the mTOR signaling pathway through DUSP2 action in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Da Ren,Wei Li,Ruijiang Zeng,Xinlin Liu,Huaiyuan Liang,Wei Xiong,Chunguang Yang,Xin Jin

Cancer biology & therapy 25:2329372 PubMed38494680

2024

CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Liu,Xiaoguang Chen,Liqi Zhang,Bo Lu

EMBO molecular medicine 16:885-903 PubMed38448544

2024

The regulatory relationship between NAMPT and PD-L1 in cancer and identification of a dual-targeting inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yang,Zefei Li,Yidong Wang,Jiwei Gao,Yangyang Meng,Simeng Wang,Xiaoyao Zhao,Chengfang Tang,Weiming Yang,Yingjia Li,Jie Bao,Xinyu Fan,Jing Tang,Jingyu Yang,Chunfu Wu,Mingze Qin,Lihui Wang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com