AB117138

ChIP Kit - One Step

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(29 제품이 사용된 논문 )

ChIP Kit ChIP Kit - One Step (ab117138) contains reagents required for chromatin immunoprecipitation (ChIP) directly from chromatin extracts isolated from mammalian cells or tissues (fresh and frozen).

- Includes a positive control antibody (RNA polymerase II), positive control (GAPDH primers) and negative control (Non-immune IgG)
- Compatible with various downstream applications including PCR (ChIP-PCR), microarrays (ChIP-chip), and sequencing (ChIP-seq)
-Enrichment ratio of positive to negative control >120, and an extremely low number of cells required (as low as 10,000 cells per ChIP reaction)
- Proven performance: cited in over 25 publications

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ChIP

Project

ChIP - ChIP Kit - One Step (AB117138)

Chromatin was prepared from HeLa cells following the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. ChIP was performed with ab8580 (Histone H3 tri-methyl K4)using ab117138. No antibody was added to the blank (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primes and probes are located in the first Kb of the transcribed region.

ChIP

Supplier Data

ChIP - ChIP Kit - One Step (AB117138)

The analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit - One Step, with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.

주요 정보

분석 소요 시간

결과 유형

Quantitative

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.</p>" } } }

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제품 세부 정보

Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.

Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

Primers

The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.

ChIP assay products and guides

Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.

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제품 구성

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab117138-96Test", "assetComponentDetails": [ { "size":"1 x 28 mL", "name":"DNA Release Buffer", "number":"AB117138-CMP04" }, { "size":"1 x 1 Unit", "name":"Sonication Frame", "number":"AB117138-CMP11" }, { "size":"1 x 1 Unit", "name":"96-Well PCR Plate", "number":"AB117138-CMP07" }, { "size":"12 x 1 Unit", "name":"8-Well Strip Caps", "number":"AB117138-CMP06" }, { "size":"1 x 12 mL", "name":"ChIP Buffer", "number":"AB117138-CMP03" }, { "size":"2 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB117138-CMP08" }, { "size":"1 x 16 µL", "name":"GAPDH Primer - Reverse (20 µM)", "number":"AB117138-CMP13" }, { "size":"1 x 16 µL", "name":"GAPDH Primer - Forward (20 µM)", "number":"AB117138-CMP12" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB117138-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB117138-CMP05" }, { "size":"1 x 16 µL", "name":"Anti-RNA Polymerase II (1 mg/ml)", "number":"AB117138-CMP02" }, { "size":"1 x 20 µL", "name":"Non-Immune IgG (1 mg/ml)", "number":"AB117138-CMP09" }, { "size":"1 x 110 µL", "name":"Proteinase K (10 mg/mL)", "number":"AB117138-CMP10" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab117138-48Test", "assetComponentDetails": [ { "size":"1 x 14 mL", "name":"DNA Release Buffer", "number":"AB117138-CMP04", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Strip Caps", "number":"AB117138-CMP06", "productcode":"" }, { "size":"1 x 8 µL", "name":"GAPDH Primer - Reverse (20 µM)", "number":"AB117138-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well PCR Plate", "number":"AB117138-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"10X Wash Buffer", "number":"AB117138-CMP05", "productcode":"" }, { "size":"1 x 8 µL", "name":"GAPDH Primer - Forward (20 µM)", "number":"AB117138-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB117138-CMP08", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB117138-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"ChIP Buffer", "number":"AB117138-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Sonication Frame", "number":"AB117138-CMP11", "productcode":"" }, { "size":"1 x 10 µL", "name":"Non-Immune IgG (1 mg/ml)", "number":"AB117138-CMP09", "productcode":"" }, { "size":"1 x 8 µL", "name":"Anti-RNA Polymerase II (1 mg/ml)", "number":"AB117138-CMP02", "productcode":"" }, { "size":"1 x 55 µL", "name":"Proteinase K (10 mg/mL)", "number":"AB117138-CMP10", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

Multi

보관 정보

Please refer to protocols

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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (29)

Recent publications for all applications. Explore the 전체 목록and refine your search

eLife 14: PubMed40464376

2025

Targeting SLC7A11-mediated cysteine metabolism for the treatment of trastuzumab-resistant HER2-positive breast cancer.

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Yijia Hua,Ningjun Duan,Chunxiao Sun,Fan Yang,Min Tian,Yanting Sun,Shuhan Zhao,Jue Gong,Qian Liu,Xiang Huang,Yan Liang,Ziyi Fu,Wei Li,Yongmei Yin

iScience 28:111614 PubMed39834855

2025

Downregulation of ECRG4 by DNMT1 promotes EC growth via IRF3/IFN-γ/miR-29b/DNMT1/ECRG4 positive feedback loop.

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Ke Yang,Shuaining Chai,Helong Song,Sinan Cao,Fangmiao Gao,Chenxuan Zhou,Linwei Li

Cell reports. Medicine 5:101806 PubMed39481387

2024

Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma.

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Junju Chen,Yixin Gao,Jian Zhong,Xujia Wu,Zhaojie Leng,Ming Liu,Yesheng Wang,Yuan Wang,Xuesong Yang,Nunu Huang,Feizhe Xiao,Maolei Zhang,Xuesong Liu,Nu Zhang

Biological research 57:27 PubMed38745315

2024

MicroRNA-721 regulates gluconeogenesis via KDM2A-mediated epigenetic modulation in diet-induced insulin resistance in C57BL/6J mice.

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Shaheen Wasil Kabeer,Shivam Sharma,Shalemraju Sriramdasu,Kulbhushan Tikoo

Cancers 16: PubMed38539453

2024

An Imbalance in Histone Modifiers Induces tRNA-Cys-GCA Overexpression and tRF-27 Accumulation by Attenuating Promoter H3K27me3 in Primary Trastuzumab-Resistant Breast Cancer.

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Ningjun Duan,Yijia Hua,Xueqi Yan,Yaozhou He,Tianyu Zeng,Jue Gong,Ziyi Fu,Wei Li,Yongmei Yin

Journal of biochemical and molecular toxicology 38:e23663 PubMed38367245

2024

l-Methionine potentiates anticancer activity of Sorafenib by epigenetically altering DUSP3/ERK pathway in hepatocellular carcinoma.

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Swagata Pal,Shaheen Wasil Kabeer,Shivam Sharma,Kulbhushan Tikoo

Cell communication and signaling : CCS 21:126 PubMed37268943

2023

LOX-1 mediates inflammatory activation of microglial cells through the p38-MAPK/NF-κB pathways under hypoxic-ischemic conditions.

Applications

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Species

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Yoshinori Aoki,Hongmei Dai,Fumika Furuta,Tomohisa Akamatsu,Takuya Oshima,Naoto Takahashi,Yu-Ichi Goto,Akira Oka,Masayuki Itoh

eLife 12: PubMed37261432

2023

Identification of Paired-related Homeobox Protein 1 as a key mesenchymal transcription factor in pulmonary fibrosis.

Applications

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Species

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Emmeline Marchal-Duval,Méline Homps-Legrand,Antoine Froidure,Madeleine Jaillet,Mada Ghanem,Deneuville Lou,Aurélien Justet,Arnaud Maurac,Aurelie Vadel,Emilie Fortas,Aurelie Cazes,Audrey Joannes,Laura Giersh,Herve Mal,Pierre Mordant,Tristan Piolot,Marin Truchin,Carine M Mounier,Ksenija Schirduan,Martina Korfei,Andreas Gunther,Bernard Mari,Frank Jaschinski,Bruno Crestani,Arnaud A Mailleux

Birth defects research 115:770-781 PubMed36899481

2023

Effects of ketamine-induced H3K9 hypoacetylation during pregnancy on cardiogenesis of mouse offspring.

Applications

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Species

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Yujuan Yu,Junjun Quan,Mou Zou,Wei Zhao,Yujuan Su,Ying Xu

Cancer science 114:2053-2062 PubMed36308276

2023

Feedforward loop between IMP1 and YAP/TAZ promotes tumorigenesis and malignant progression in glioblastoma.

Applications

Unspecified application

Species

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Jia Yang,Xujia Wu,Jia Wang,Xing Guo,Junju Chen,Xuesong Yang,Jian Zhong,Xixi Li,Zhong Deng

제품이 사용된 논문 모두 보기

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

ChIP Kit - One Step

ChIP - ChIP Kit - One Step (AB117138)

ChIP - ChIP Kit - One Step (AB117138)

주요 정보

분석 소요 시간

결과 유형

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Sample Prep & Detection Kits

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (29)

Product promise