AB185913

High-Sensitivity ChIP Kit

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High-Sensitivity ChIP kit (ab185913) is a complete set of optimized reagents to carry out a successful chromatin immunoprecipitation (CoIP) procedure in a high throughput format starting from mammalian cells or tissues.

- Proven performance: cited in over 50 publications
- Optimized to reduce non-specific background
- Enables detection of low-abundance targets
- Compatible with ChIP-PCR, microarrays & sequencing (ChIP-seq)

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ChIP - High-Sensitivity ChIP Kit (AB185913)

ChIP

Supplier Data

ChIP - High-Sensitivity ChIP Kit (AB185913)

High abundance protein enrichment : .

Sheared chromatin isolated from different numbers of MBD-231 cells was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters using ab185913 and a quantitative PCR Fast Kit.

ChIP

Supplier Data

ChIP - High-Sensitivity ChIP Kit (AB185913)

Low abundance protein enrichment : .

Sheared chromatin isolated from different numbers of MCF7 cells was used for ChIP-qPCR analysis of ER-a enrichment in TFF1 promoters using ab185913 and a quantitative PCR Fast Kit.

ChIP-sequencing - High-Sensitivity ChIP Kit (AB185913)

ChIP-seq

Supplier Data

ChIP-sequencing - High-Sensitivity ChIP Kit (AB185913)

Histone H3K18ac ChIP assay example data.

Histone H3K18ac ChIP assay was carried out using ab185913.

ChIP-Seq reads align with the same peak sites as ENCODE data.

주요 정보

샘플 타입

Adherent cells, Suspension cells, Tissue

Reacts with

Mammals

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.</p>" } } }

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제품 세부 정보

High-Sensitivity ChIP Kit (ab185913) is a complete set of optimized reagents to carry out a successful chromatin immunoprecipitation procedure in a high throughput format starting from mammalian cells or tissues. The highly specific and sensitive kit is suitable for selective enrichment of a chromatin fraction containing specific DNA sequences using various mammalian cell/tissues. The optimized protocol and kit components reduce non-specific background ChIP levels to allow capture of low abundance protein/transcription factors and increased specific enrichment of target protein/DNA complexes. The target protein bound DNA prepared with the High-Sensitivity ChIP Kit can be used for various downstream applications including PCR (ChIP-PCR), microarrays (ChIP-on-chip), and sequencing (ChIP-seq).

Starting Materials
Starting materials can include various tissue or cell samples such as cells from flask or plate cultured cells, fresh and frozen tissues, etc. In general, the amount of cells and tissues for each reaction can be 2 x 10³ to 1 x 10⁶ and 0.5 mg to 50 mg, respectively. For optimal preparation, the input amount should be 1-2 x 10⁵ cells or 10-20 mg tissues since the enrichment of target proteins to genome loci may vary. For the target proteins that are low abundance transcription factors, the input amount should be 5-6 x 10⁵ cells or 50 to 60 mg tissues.

Primers
The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.

Protein-DNA interaction plays a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction is important for understanding cellular processes. Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying such protein-DNA interactions. It allows for the detection of a specific protein bound to a specific gene sequence in living cells using PCR (ChIP-PCR), microarrays (ChIP-chip), or sequencing (ChIP-seq). For example, measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while the recruitment of methylated H3-K9 to the promoters on a genome-wide scale can be detected by ChIP-on-chip or ChIP-sequencing. ChIP analysis requires that ChIPed DNA contains minimal background in order to reliably identify true TF-enriched regions. High background in ChIP is mainly caused ineffective wash buffers, insufficient cross-link reversal, inappropriate DNA fragment length, and residual RNA interference. To effectively capture TF/DNA complexes, which are often in low abundance, an ideal ChIP method requires having maximum sensitivity with minimized background levels. This method should also be able to enrich highly abundant protein/DNA complexes using a small amount of cells or tissues in a high throughput format. The High-Sensitivity ChIP Kit is designed to achieve these goals by maximizing sensitivity and minimizing non-specific background signals.

ChIP assay products and guides

Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

Multi

적절한 장기 보관 조건

Multi

보관 정보

Please refer to protocols

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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (52)

Recent publications for all applications. Explore the 전체 목록and refine your search

Journal of neuroinflammation 22:13 PubMed39844302

2025

Therapeutic potential of targeting the IRF2/POSTN/Notch1 axis in nucleus pulposus cells for intervertebral disc degeneration.

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Daxue Zhu,Zhaoheng Wang,Shijie Chen,Yanhu Li,Xuewen Kang

Cellular and molecular gastroenterology and hepatology 19:101422 PubMed39419394

2024

Hepatitis B Virus-KMT2B Integration Drives Hepatic Oncogenic Processes in a Human Gene-edited Induced Pluripotent Stem Cells-derived Model.

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Jun Tsuchiya,Masato Miyoshi,Sei Kakinuma,Fukiko Kawai-Kitahata,Akihide Kamiya,Taro Shimizu,Ayako Sato,Keiya Watakabe,Tomohiro Mochida,Kento Inada,Rion Kamimae,Shun Kaneko,Miyako Murakawa,Sayuri Nitta,Mina Nakagawa,Mamoru Watanabe,Yasuhiro Asahina,Ryuichi Okamoto

Nature communications 15:8114 PubMed39284834

2024

Erythropoietin regulates energy metabolism through EPO-EpoR-RUNX1 axis.

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Species

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Weiqin Yin,Praveen Kumar Rajvanshi,Heather M Rogers,Teruhiko Yoshida,Jeffrey B Kopp,Xiuli An,Max Gassmann,Constance T Noguchi

Cell death & disease 15:559 PubMed39097593

2024

Enzalutamide inhibits PEX10 function and sensitizes prostate cancer cells to ROS activators.

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Species

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Yuankang Feng,Yu Zhang,Hao Li,Tao Wang,Fubo Lu,Ruoyang Liu,Guoqing Xie,Liang Song,Budeng Huang,Xiang Li,Yinghui Ding,Jinjian Yang,Zhankui Jia,Zhenlin Huang

Cancer science 115:3288-3304 PubMed39054797

2024

The molecular mechanism underlying KRAS regulation on STK31 expression in pancreatic ductal adenocarcinoma.

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Yuting Liu,Shing Chun Tang,Chi Han Li,Ka Fai To,Bo Li,Stephen Lam Chan,Chi Hin Wong,Yangchao Chen

Cell death & disease 15:435 PubMed38902235

2024

ALKBH4 impedes 5-FU Sensitivity through suppressing GSDME induced pyroptosis in gastric cancer.

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Species

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Xin Jiang,Zhiman Zhu,Lina Ding,Wenqi Du,Dongsheng Pei

Molecular medicine (Cambridge, Mass.) 30:84 PubMed38867168

2024

HOXD9 regulated mitophagy to promote endothelial progenitor cells angiogenesis and deep vein thrombosis recanalization and resolution.

Applications

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Species

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Zhang Xiujin,Guo Lili,Fan Jing,Ye Wenhai,Liu Sikai,Shi Wan-Yin

Molecular medicine (Cambridge, Mass.) 30:53 PubMed38649840

2024

Elk1 enhances inflammatory cell infiltration and exacerbates acute lung injury/acute respiratory distress syndrome by suppressing Fcgr2b transcription.

Applications

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Species

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Shiyou Wei,Dandan Ling,Jingui Zhong,Rui Chang,Xinyu Ling,Zhigang Chen,Ruowang Duan

Frontiers in aging neuroscience 15:1326127 PubMed38192280

2024

O-GlcNAc regulates the mitochondrial integrated stress response by regulating ATF4.

Applications

Unspecified application

Species

Unspecified reactive species

Ibtihal M Alghusen,Marisa S Carman,Heather Wilkins,Sophiya John Ephrame,Amy Qiang,Wagner B Dias,Halyna Fedosyuk,Aspin R Denson,Russell H Swerdlow,Chad Slawson

Cell death & disease 14:767 PubMed38007473

2023

LINC01133 can induce acquired ferroptosis resistance by enhancing the FSP1 mRNA stability through forming the LINC01133-FUS-FSP1 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Shaowen Wang,Jionghuang Chen,Pengping Li,Yangchao Chen

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High-Sensitivity ChIP Kit

ChIP - High-Sensitivity ChIP Kit (AB185913)

ChIP - High-Sensitivity ChIP Kit (AB185913)

ChIP-sequencing - High-Sensitivity ChIP Kit (AB185913)

주요 정보

샘플 타입

Reacts with

보완 제품

Secondary Antibodies

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Secondary Antibodies

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (52)

Product promise