5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30

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5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (ab270709) enables simple and rapid conjugation of oligonucleotide barcodes to antibodies to enable cell surface protein analysis in Multiomic Cytometry on 10x Genomics Chromium Controller. This kit is compatible with Chromium Single Cell Immune Profiling.

The kit includes 10 distinct oligonucleotide barcodes ready to conjugate to 10 μg of antibody each. The protocol involves only a few steps, requires minimal hands-on time and doesn't require any expertise in antibody conjugation or oligonucleotide sourcing.

Please find antibody buffer considerations and compatibility in the protocol booklet. For incompatible buffers and low antibody concentrations, please see our antibody purification and concentration kits.

For conjugating antibodies with additional distinct oligonucleotide barcodes, please see 5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (ab270703) and 5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (ab270705). 100 μg conjugation kits are available on-demand for each of these 30 oligonucleotide barcodes. For conjugating more than 30 antibodies with distinct oligonucleotide barcodes, please contact us to discuss your requirements.

For oligonucleotide barcode sequence information, please see appendix in the protocol booklet.

Storing and handling conjugation kits

Lyophilized Lightning-Link^® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.