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AB201806

DyLight® 800 Conjugation Kit (Fast) - Lightning-Link®

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(6 제품이 사용된 논문 )

DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (ab201806) offers several standout features:

- Rapid Conjugation: achieve DyLight® 800 labeling in under 20 minutes with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides. DyLight® 800-labeled antibodies can be used immediately in applications such as Western blot, ELISA, and Immunohistochemistry (IHC) without further purification.
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Schematic Diagram - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)
  • Schematic Diagram

Supplier Data

Schematic Diagram - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)

This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab201806 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

Western blot - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)
  • WB

PubMed

Western blot - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)

Western blot - DyLight 800 Conjugation Kit (Fast) Lightning-Link.

Ou, Li et al used DyLight® 800 Conjugation Kit - Lightning-Link® (ab201806) as part of examining murine Hurler syndrome. They used the kit to conjugate DyLight® 800 to anti-GAPDH antibody for use in western blot.
In Vivo ZFN-Mediated Insertion of hIDUA at the Albumin Locus Results in Expression of Active Enzyme in MPS I Mouse Hepatocytes. Expression of hIDUA in mouse liver protein extracts 1 or 4 months post-treatment. Each lane represents an individual mouse. GAPDH is shown as a loading control.

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Image from Ou, Li et al. Molecular therapy: the journal of the American Society of Gene Therapy vol. 27,1 (2019): 178-187. doi:10.1016/j.ymthe.2018.10.018 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Other - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)
  • Other

Lab

Other - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)

Representative image of DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (ab201806)

Components shown from left to right :

DyLight® 800 mix (3 vials for 3x 10 µg, 3x 100 µg)

Modifier reagent (1 vial)

Quencher reagent (1 vial)

Securitainer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the conjugation kit.

Note : Color of the lyophilized material in image may not precisely match the color shade in the actual kit.

Other - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)
  • Other

Lab

Other - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)

Representative image of DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (ab201806)

Components shown from left to right :

DyLight® 800 mix (1 vial for 100 µg, 1 mg)

Modifier reagent (1 vial)

Quencher reagent (1 vial)

Securitainer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the conjugation kit.

Note : Color of the lyophilized material in image may not precisely match the color shade in the actual kit.

주요 정보

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보완 제품

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제품 세부 정보

DyLight® 800 Conjugation Kit / DyLight® 800 Labeling Kit (ab201806) uses a simple and quick process for DyLight 800 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.

To conjugate an antibody to DyLight® 800 using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The DyLight® 800 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.

Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to DyLight® 800.

Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid DyLight® 800 Labeling Kit. 329-0005 is the same as the 100 μg size. 329-0010 is the same as the 3 x 100 ug size. 329-0030 is the same as the 3 x 10 ug size. 329-0015 is the same as the 1 mg size.

Amount and volume of antibody for conjugation to DyLight® 800

Kit size Recommended
amount of antibody1		 Maximum
amount of antibody		 Maximum antibody
volume2
3 x 10 μg 3 x 10 μg 3 x 20 μg 3 x 10 μL
100 μg 1 x 100 μg 1 x 200 μg 1 x 100 μL
3 x 100 μg 3 x 100 μg 3 x 200 μg 3 x 100 μL
1 mg 1 x 1 mg 1 x 2 mg 1 x 1 mL

1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1 mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

50mM / 0.6% Tris1 0.1% BSA2 50% glycerol
0.1% sodium azide PBS Potassium phosphate
Sodium chloride HEPES Sucrose
Sodium citrate EDTA Trehalose

1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.

Incompatible buffer constituents

Thiomerosal Proclin Glycine
Arginine Glutathione DTT

If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Storing and handling conjugation kits

Lyophilized Lightning-Link® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Ambient - Cannot Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer cell 40:1060-1069.e7 PubMed36099883

2022

A covalent inhibitor of K-Ras(G12C) induces MHC class I presentation of haptenated peptide neoepitopes targetable by immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Zhang,Peter J Rohweder,Chayanid Ongpipattanakul,Koli Basu,Markus-Frederik Bohn,Eli J Dugan,Veronica Steri,Byron Hann,Kevan M Shokat,Charles S Craik

Journal of orthopaedic translation 35:72-80 PubMed36186660

2022

Loss of Bcl-3 delays bone fracture healing through activating NF-κB signaling in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fuxiao Wang,Jiawei Guo,Yili Wang,Yan Hu,Hao Zhang,Jiao Chen,Yingying Jing,Liehu Cao,Xiao Chen,Jiacan Su

eLife 9: PubMed32746966

2020

Expression of different L1 isoforms of papillomavirus as mechanism to circumvent adaptive immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Fu,Rui Cao,Miriam Schäfer,Sonja Stephan,Ilona Braspenning-Wesch,Laura Schmitt,Ralf Bischoff,Martin Müller,Kai Schäfer,Sabrina E Vinzón,Frank Rösl,Daniel Hasche

Molecular therapy. Methods & clinical development 18:607-619 PubMed32775495

2020

AAV2/6 Gene Therapy in a Murine Model of Fabry Disease Results in Supraphysiological Enzyme Activity and Effective Substrate Reduction.

Applications

Unspecified application

Species

Unspecified reactive species

Makiko Yasuda,Marshall W Huston,Silvere Pagant,Lin Gan,Susan St Martin,Scott Sproul,Daniel Richards,Stephen Ballaron,Khaled Hettini,Annemarie Ledeboer,Lillian Falese,Liching Cao,Yanmei Lu,Michael C Holmes,Kathleen Meyer,Robert J Desnick,Thomas Wechsler

Molecular therapy : the journal of the American Society of Gene Therapy 27:178-187 PubMed30528089

2018

ZFN-Mediated In Vivo Genome Editing Corrects Murine Hurler Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ou,Russell C DeKelver,Michelle Rohde,Susan Tom,Robert Radeke,Susan J St Martin,Yolanda Santiago,Scott Sproul,Michael J Przybilla,Brenda L Koniar,Kelly M Podetz-Pedersen,Kanut Laoharawee,Renee D Cooksley,Kathleen E Meyer,Michael C Holmes,R Scott McIvor,Thomas Wechsler,Chester B Whitley

Molecular therapy : the journal of the American Society of Gene Therapy 26:1127-1136 PubMed29580682

2018

Dose-Dependent Prevention of Metabolic and Neurologic Disease in Murine MPS II by ZFN-Mediated In Vivo Genome Editing.

Applications

Unspecified application

Species

Unspecified reactive species

Kanut Laoharawee,Russell C DeKelver,Kelly M Podetz-Pedersen,Michelle Rohde,Scott Sproul,Hoang-Oanh Nguyen,Tam Nguyen,Susan J St Martin,Li Ou,Susan Tom,Robert Radeke,Kathleen E Meyer,Michael C Holmes,Chester B Whitley,Thomas Wechsler,R Scott McIvor
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