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AB204530

24(S)-Hydroxycholesterol ELISA Kit

2

(2 리뷰들)

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(5 제품이 사용된 논문 )

24(S)-Hydroxycholesterol ELISA Kit is a competitive ELISA designed to quantify 24(S)-Hydroxycholesterol with a sensitivity of 0.78 ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 0.39 - 100 ng/ml
2 이미지
ELISA - 24(S)-Hydroxycholesterol ELISA Kit (AB204530)
  • ELISA

Supplier Data

ELISA - 24(S)-Hydroxycholesterol ELISA Kit (AB204530)

Standard Curve

ELISA - 24(S)-Hydroxycholesterol ELISA Kit (AB204530)
  • ELISA

Supplier Data

ELISA - 24(S)-Hydroxycholesterol ELISA Kit (AB204530)

Dose Response Curve

주요 정보

검출 방식

Colorimetric

샘플 타입

Cerebral Spinal Fluid, Tissue Homogenate, Tissue Culture Media

분석 유형

Competitive

Sensitivity

= 0.78 ng/mL

검출 범위

0.39 - 100 ng/mL

분석 소요 시간

2h

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Assay Kits

AB65359

Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation

Biochemical assay - Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (AB65359)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's 24(S)-Hydroxycholesterol (24-OHC) kit (ab204530) is an in-vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of 24-OHC levels in tissue culture media, cerebral spinal fluid and tissue homogenate samples.

The homeostasis and trafficking of cholesterol is an essential component of both the central and peripheral nervous system in the maintenance of neuronal tissues. Disturbances in this homeostasis may be due to the onset of various neurological diseases such as Alzheimer's Disease, Huntington's Disease and multiple sclerosis.

Apolipoprotein E and Cyp46 (also known as 24S-Cholesterol Hydroxylase) are both important in the homeostasis of cerebral cholesterol 6 and thus are of clinical interest in understanding the relation of these molecules with the pathogenesis of these, and potentially other, neurodegenerative diseases.

24-OHC, an enzymatically-generated side chain-hydroxylated derivative of cholesterol, is a pivotal marker in the study of cerebral cholesterol homeostasis. Cholesterol is unable to cross the blood-brain barrier however, Cyp46 enzyme converts cholesterol to the more soluble 24-OHC, and this hydroxylated form of cholesterol is able to cross the bloodbrain barrier.7,8 This conversion allows for the reduction of cholesterol in the brain and the efflux of 24-OHC from the brain into cerebral spinal fluid and blood. The flux of 24-OHC has been observed in patients with a variety of neurodegenerative diseases. In the instance of Alzheimer's disease, the change in 24S-hydroxycholesterol concentrations may be indicative of different pathogenetic mechanisms and/or the progression of the disease.3 As in the case of multiple sclerosis, concentrations of 24-OHC have been shown to decrease, likely due to the loss of neuronal cells responsible for the synthesis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Buffer 1", "replicates": 24, "mean": "28.2 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 9.3" }, { "reproducibilityType": "Inter", "sample": "Buffer 2", "replicates": 10, "mean": "11.1 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 18.5" }, { "reproducibilityType": "Intra", "sample": "Buffer 2", "replicates": 24, "mean": "9 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Inter", "sample": "Buffer 1", "replicates": 10, "mean": "28.6 ng/mL", "standardDeviation": null, "coefficientOfVariability": "= 18.4" } ]
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Recovery

[ { "sample": "Tissue Culture Media", "range": null, "average": "= 101.7" }, { "sample": "Cerebral Spinal Fluid", "range": null, "average": "= 99" }, { "sample": "Tissue Homogenate", "range": null, "average": "= 98.6" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab204530-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Goat anti-Rabbit IgG coated microplate (12x 8 well strips)", "number":"AB204530-CMP10", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Plate Sealer", "number":"AB204530-CMP02", "productcode":"" }, { "size":"2 x 10 mL", "name":"TMB Substrate", "number":"AB204530-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution (1N Soln.)", "number":"AB204530-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Assay Buffer 40", "number":"AB204530-CMP01", "productcode":"" }, { "size":"1 x 70 µL", "name":"24(S)-Hydroxycholesterol Standard", "number":"AB204530-CMP08", "productcode":"" }, { "size":"1 x 27 mL", "name":"Wash Buffer Concentrate (20X Conc.)", "number":"AB204530-CMP09", "productcode":"" }, { "size":"1 x 70 µL", "name":"24(S)-Hydroxycholesterol Conjugate (100X Conc.)", "number":"AB204530-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Streptavidin-HRP (1X Soln.)", "number":"AB204530-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"24(S)-Hydroxycholesterol Antibody (1X Soln.)", "number":"AB204530-CMP06", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cholesterol sometimes referred to as fchol is a lipid molecule with a molecular mass of approximately 386.65 Daltons. It is an essential component of cell membranes and is found in the plasma membranes of cells throughout the body. Cholesterol primarily resides in the membranes where it modulates fluidity and mechanical properties. Its expression occurs in various tissues but it is abundant in the liver which plays an important role in its synthesis and regulation.
Biological function summary

Cholesterol is important in maintaining the structural integrity and organization of membranes. It is also part of lipid rafts which are specialized microdomains within the cell membrane that facilitate cellular signaling. Cholesterol interacts with proteins and receptors embedded in the membrane influencing their function. It does not form a complex itself but affects complexes within the membrane like lipid rafts and signaling platforms.

Pathways

Cholesterol is a critical component of the steroid biosynthesis pathway and the bile acid synthesis pathway. It serves as a precursor for steroid hormones including cortisol and sex hormones as well as bile acids which are essential for digestion and lipid absorption. Proteins such as HMG-CoA reductase and the low-density lipoprotein receptor (LDLR) are key players related to cholesterol in these pathways regulating its synthesis and uptake in cells.

Cholesterol is closely associated with atherosclerosis and cardiovascular disease. Elevated levels of low-density lipoprotein (LDL) cholesterol contribute to plaque formation in arteries increasing the risk of heart attack and stroke. On the other hand high-density lipoprotein (HDL) cholesterol helps remove excess cholesterol from tissues including the arterial walls. Statins a class of drugs that lower LDL cholesterol levels target HMG-CoA reductase and are commonly used in treating hypercholesterolemia and cardiovascular diseases.
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제품 프로토콜

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타겟 정보

See full target information 24(S)-hydroxycholesterol

대체 명칭 보기

IMD42, LXR a, Liver X receptor alpha, MGC129539, NR1F3, NR1H3_HUMAN, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Nuclear receptor subfamily 1 group H member 3, Oxysterols receptor LXR-alpha, RAR related orphan nuclear receptor variant 2, RAR related orphan receptor C, isoform a, RAR related orphan receptor gamma, RAR-related orphan receptor C, RLD 1, RORG_HUMAN, RZR GAMMA, RZRG, Retinoic acid binding receptor gamma, Retinoid-related orphan receptor-gamma, Rorc, TOR
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 10: PubMed40540390

2025

Treatment with efavirenz extends survival in a Creutzfeldt-Jakob disease model by regulating brain cholesterol metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tahir Ali,Jessica Cashion,Samia Hannaoui,Hanaa Ahmed-Hassan,Hermann Schatzl,Sabine Gilch

PloS one 17:e0273206 PubMed35998127

2022

Maternal stress induced anxiety-like behavior exacerbated by electromagnetic fields radiation in female rats offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Ehsan Hosseini,Mahsa Farid Habibi,Shirin Babri,Gisou Mohaddes,Hajar Abkhezr,Hamed Heydari

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 41:312-323 PubMed32169014

2020

Serum 24S-hydroxycholesterol predicts long-term brain structural and functional outcomes after hypoxia-ischemia in neonatal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fuxin Lu,Shujuan Fan,Andrea R Romo,Duan Xu,Donna M Ferriero,Xiangning Jiang

EMBO reports 20:e48143 PubMed31535451

2019

NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel N Mitroi,Guadalupe Pereyra-Gómez,Beatriz Soto-Huelin,Fernando Senovilla,Toshihide Kobayashi,Jose A Esteban,María Dolores Ledesma

Pediatric research 83:1218-1227 PubMed29718007

2018

Upregulation of cholesterol 24-hydroxylase following hypoxia-ischemia in neonatal mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Fuxin Lu,Jun Zhu,Selena Guo,Brandon J Wong,Farid F Chehab,Donna M Ferriero,Xiangning Jiang
제품이 사용된 논문 모두 보기
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