AB133043

8-iso-PGF2alpha ELISA Kit

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

8-iso-PGF2alpha ELISA Kit is a Competitive ELISA for the measurement of 8-iso-PGF2alpha in in Biofluids, Cell/Tissue Extracts samples.

2 이미지

Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)

cELISA

Supplier Data

Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)

Typical Standard Curve - Overnight Format.

Representative Standard Curve using ab133043. Overnight Format.

cELISA

Supplier Data

Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)

Typical Standard Curve - 2 Hour Format.

Representative Standard Curve using ab133043. 2 Hour Format.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Tissue, Serum

분석 유형

Competitive

Sensitivity

= 40 pg/mL

검출 범위

160 - 100000 pg/mL

분석 소요 시간

분석 플랫폼

Microplate

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

제품 세부 정보

Abcam's Direct 8-iso-PGF₂alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 8-iso-PGF_2α in Biological fluids.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-8-iso-PGF₂alpha antigen and a rabbit polyclonal antibody specific to 8-iso-PGF₂alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 8-iso-PGF₂alpha captured in the plate.

The 8-epimer of Prostaglandin F_2α (8-iso-PGF_2α) is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependent mechanisms from arachidonic acid. 8-iso-PGF_2α has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and atherosclerosis and a mutagen in 3T3 cells and in vascular smooth muscle cells. It has also been postulated to participate as a pathophysiological mediator and is able to modify the fluidity and integrity of membranes. 8-isoPGF_2α has been shown to circulate in plasma and is excreted in urine. Methods for assessing total 8-iso-PGF_2α typically require the alkaline hydrolysis of 8-iso-PGF_2α esters from tissues, followed by length procedures involving extractions, phase separations and thin layer chromatography.

Cross Reactivity

Compound	% Cross Reactivity
8-iso-PGF_2α	100
PGF_1α	4.6
PGF_2α	1.85
PGE₁	0.19
TXB₂	0.023
PGB₁	0.02
PGE₃	0.012
6-keto-PGF_1α	0.008
13,14-dihydro-15-keto-PGF_2α	0.008
6,15-keto-13,14-dihydro-PGF_1α	0.005
8-iso-PGE₁	<0.001
PGA₂	<0.001
PGJ₂	<0.001
2-Arachidonoylglycerol	<0.001
Anandamide	<0.001

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "1473 pg/mL Spike", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Inter", "sample": "4501 pg/mL Spike", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Inter", "sample": "6679 pg/mL Spike", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" }, { "reproducibilityType": "Intra", "sample": "3264 pg/mL Spike", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "5092 pg/mL Spike", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" }, { "reproducibilityType": "Intra", "sample": "970 pg/mL Spike", "replicates": 16, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.3" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": null, "average": "= 93.8" }, { "sample": "EDTA Plasma", "range": null, "average": "= 109.8" }, { "sample": "Heparin Plasma", "range": null, "average": "= 97.2" } ]

style

left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133043-1x96Tests", "assetComponentDetails": [ { "size":"1 x 0.5 mL", "name":"8-iso-PGF2 alpha Standard", "number":"AB133043-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"pNpp Substrate", "number":"AB133043-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB133043-CMP04", "productcode":"" }, { "size":"1 x 27 mL", "name":"Direct 8-iso-PGF2 alpha Sample Diluent", "number":"AB133043-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Sealer", "number":"AB133043-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"Neutralizing Reagent", "number":"AB133043-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Goat anti-rabbit IgG Microplate (12 x 8 wells)", "number":"AB133043-CMP10", "productcode":"" }, { "size":"1 x 27 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133043-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"Direct 8-iso-PGF2 alpha Antibody", "number":"AB133043-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"Direct 8-iso-PGF2 alpha Alkaline Phosphatase Conjugate", "number":"AB133043-CMP06", "productcode":"" } ] } } }

style

left-column

특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

8-iso-PGF2α also known as 8-iso-PGF2a iso-8-PGF2 or PGF2 alpha is an isoprostane formed by the free-radical-catalyzed peroxidation of arachidonic acid. It has a mass of approximately 354 g/mol. This molecule is commonly found in cell membranes during oxidative stress. It exists in various tissues with higher expressions noted in the liver heart and brain. 8-iso-PGF2α can be detected using methods such as the Alpha ELISA or Iso Kit which are critical for measuring its levels in biological samples.

Biological function summary

8-iso-PGF2α functions as a marker of oxidative stress within the organism. It participates in cell signaling affecting vascular tone and platelet aggregation. It does not form part of a larger protein complex by itself but acts through receptor-mediated mechanisms mainly interacting with thromboxane receptors to exert its effects. This interaction leads to vasoconstriction and plays roles in various cell signaling pathways.

Pathways

8-iso-PGF2α is involved in the oxidative stress response and the arachidonic acid metabolism pathways. It closely relates to the cyclooxygenase (COX) pathway intersecting with proteins such as COX-2 and modulating inflammatory responses. This interaction can influence the production of other prostanoids which further integrate into the inflammatory response pathways.

8-iso-PGF2α serves as a biomarker for conditions where oxidative stress is increased such as atherosclerosis and Alzheimer's disease. It connects to diseases through its impact on oxidative stress markers and its influence on vascular health. The molecule's interaction with thromboxane receptors and COX-2 makes it relevant in the progression of these diseases highlighting its potential as a therapeutic target for modulating oxidative damage in related conditions.

style

left-column

제품 프로토콜

style

left-column

타겟 정보

See full target information 8-iso-PGF2a

대체 명칭 보기

8-iso-Prostaglandin F2a

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Arteriosclerosis, thrombosis, and vascular biology 39:2303-2319 PubMed31510791

2019

ALDH2 (Aldehyde Dehydrogenase 2) Protects Against Hypoxia-Induced Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhao,Bailu Wang,Jian Zhang,Dayu He,Qun Zhang,Chang Pan,Qiuhuan Yuan,Yinan Shi,Haiyang Tang,Feng Xu,Shujian Wei,Yuguo Chen

Journal of diabetes investigation 10:1189-1198 PubMed30825261

2019

Glycine increases glyoxalase-1 function by promoting nuclear factor erythroid 2-related factor 2 translocation into the nucleus of kidney cells of streptozotocin-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Wang,Dan Zhao,Lei Chen,Jingjing Li,Geheng Yuan,Guosheng Yang,Hong Zhang,Xiaohui Guo,Junqing Zhang

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

8-iso-PGF2alpha ELISA Kit

Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)

Competitive ELISA - 8-iso-PGF2alpha ELISA Kit (AB133043)

주요 정보

검출 방식

샘플 타입

분석 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise