AB108794

Albumin Pig ELISA Kit

Name: Albumin Pig ELISA Kit
Brand: Abcam Limited
SKU: AB108794
Rating: 4 (1 reviews)

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(3 제품이 사용된 논문 )

Albumin Pig ELISA Kit is a Sandwich ELISA for the measurement of Albumin Pig in Pig in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

1 이미지

Sandwich ELISA - Albumin Pig ELISA Kit (AB108794)

sELISA

Supplier Data

Sandwich ELISA - Albumin Pig ELISA Kit (AB108794)

Representative Standard Curve using ab108794

주요 정보

검출 방식

Colorimetric

샘플 타입

Urine, Cell culture supernatant, Cell Lysate, Tissue

Reacts with

Pig

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.37 ng/mL

검출 범위

1.563 - 100 ng/mL

분석 소요 시간

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Abcam's Albumin Pig in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of pig Albumin levels in urine, cell culture supernatant, cell lysate, and tissue samples.

An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108794-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Albumin Microplate (12 x 8 well strips)", "number":"AB108794-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108794-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108794-CMP05", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108794-CMP03", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108794-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108794-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108794-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Pig Albumin Antibody", "number":"AB108794-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Albumin Standard", "number":"AB108794-CMP08", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

Multi

보관 정보

Please refer to protocols

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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제품 프로토콜

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타겟 정보

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).

See full target information ALB

대체 명칭 보기

Albumin, ALB

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 24: PubMed37895114

2023

Adipose Tissue-Derived Mesenchymal Stem Cells Extend the Lifespan and Enhance Liver Function in Hepatocyte Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Sun A Ock,Seo-Yeon Kim,Won Seok Ju,Young-Im Kim,Ha-Yeon Wi,Poongyeon Lee

Theranostics 11:7620-7639 PubMed34335954

2021

Fiber scaffold bioartificial liver therapy relieves acute liver failure and extrahepatic organ injury in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Weng,Xu Han,Fanhong Zeng,Yue Zhang,Lei Feng,Lei Cai,Kangyan Liang,Shusong Liu,Shao Li,Gongbo Fu,Min Zeng,Yi Gao

Cell transplantation 26:1840-1854 PubMed29390876

2018

Decellularization and Solubilization of Porcine Liver for Use as a Substrate for Porcine Hepatocyte Culture: Method Optimization and Comparison.

Applications

Unspecified application

Species

Unspecified reactive species

Ramon E Coronado,Maria Somaraki-Cormier,Shanmugasundaram Natesan,Robert J Christy,Joo L Ong,Glenn A Halff

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Albumin Pig ELISA Kit

Sandwich ELISA - Albumin Pig ELISA Kit (AB108794)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise