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AB205069

Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit

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Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit is a Sandwich ELISA for the measurement of Bovine Alpha 1 Acid Glycoprotein / AGP in Cow in Biofluids samples.
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Sandwich ELISA - Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB205069)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB205069)

Representative standard curve using ab205069 Bovine Alpha 1 Acid Glycoprotein / AGP ELISA kit.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Serum

Reacts with

Cow

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.706 ng/mL

검출 범위

3.125 - 100 ng/mL

분석 소요 시간

1h 40m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Primary Antibodies

AB10483

Anti-KAP1 antibody

Immunoprecipitation - Anti-KAP1 antibody (AB10483)

  • 3
  • 7
Assay Kits

AB284009

Human KAP1 ELISA Kit

Sandwich ELISA - Human KAP1 ELISA Kit (AB284009)

  • 0
  • 0
Primary Antibodies

AB239358

Anti-KMT4 / Dot1L antibody [EPR22936-138]

BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT4 / Dot1L antibody [EPR22936-138] (AB239358)

  • 0
  • 0
Assay Kits

AB313916

Human Osteocrin ELISA Kit

Sandwich ELISA - Human Osteocrin ELISA Kit (AB313916)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Alpha 1 Acid Glycoprotein / AGP in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Alpha 1 Acid Glycoprotein / AGP protein in bovine biological fluids.

In this assay the Alpha 1 Acid Glycoprotein / AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein / AGP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein / AGP antibodies conjugated with bovineradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein / AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein / AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein / AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein / AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.
Biological function summary

AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.

Pathways

Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.

AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.
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제품 프로토콜

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타겟 정보

See full target information ORM1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 14:1091066 PubMed36793724

2023

Application of quantitative proteomics to discover biomarkers for tick resistance in cattle.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Raza,Benjamin L Schulz,Amanda Nouwens,Muhammad Noman Naseem,Muhammad Kamran,Emily F Mantilla Valdivieso,Edward D Kerr,Constantin Constantinoiu,Nicholas N Jonsson,Peter James,Ala E Tabor
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

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