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AB323740

Bovine Beta-Lactoglobulin (LGB) ELISA Kit

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Bovine Beta-Lactoglobulin (LGB) ELISA Kit is a Sandwich ELISA for the measurement of Bovine Beta-Lactoglobulin (LGB) in Cow in Biofluids samples.
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Sandwich ELISA - Bovine Beta-Lactoglobulin (LGB) ELISA Kit (AB323740)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine Beta-Lactoglobulin (LGB) ELISA Kit (AB323740)

Example of bovine Beta-Lactoglobulin standard curve in Sample Diluent NS + Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Bovine Beta-Lactoglobulin (LGB) ELISA Kit (AB323740)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine Beta-Lactoglobulin (LGB) ELISA Kit (AB323740)

Example of bovine Beta-Lactoglobulin standard curve in Sample Diluent NS + Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Bovine Beta-Lactoglobulin (LGB) ELISA Kit (AB323740)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine Beta-Lactoglobulin (LGB) ELISA Kit (AB323740)

Interpolated concentration of native Beta-Lactoglobulin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : pasteurized milk 1 : 800,000, ultra-pasteurized milk 1 : 100,000, and reconstituted powdered dry milk 1 : 200,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + Enhancer.

Sandwich ELISA - Bovine Beta-Lactoglobulin (LGB) ELISA Kit (AB323740)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine Beta-Lactoglobulin (LGB) ELISA Kit (AB323740)

Interpolated concentration of native Beta-Lactoglobulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 800,000 pasteurized milk. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + Enhancer.

주요 정보

검출 방식

Colorimetric

샘플 타입

Milk

Reacts with

Cow

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 215.6 pg/mL

검출 범위

625 - 10000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

Bovine Beta-Lactoglobulin (LGB) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta-Lactoglobulin (LGB) protein in Milk. Quantitate Bovine Beta-Lactoglobulin (LGB) with 215.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

Precision

[ { "reproducibilityType": "Intra", "sample": "Milk", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.4" }, { "reproducibilityType": "Inter", "sample": "Milk", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.4" } ]

Recovery

[ { "sample": "Milk", "range": "105 - 109 %", "average": "= 107" } ]

제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C

제품 프로토콜

타겟 정보

Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
See full target information LGB

대체 명칭 보기

Beta-lactoglobulin, Beta-LG, LGB
websiteProtocolBooklet
en

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