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AB273191

Bovine IFN-alpha ELISA Kit

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Bovine IFN-alpha ELISA Kit is a Sandwich ELISA for the measurement of Bovine IFN-alpha in Cow in Biofluids, Cell Culture Media samples.

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Interferon alpha-1

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Sandwich ELISA - Bovine IFN-alpha ELISA Kit (AB273191)
  • sELISA

Supplier Data

Sandwich ELISA - Bovine IFN-alpha ELISA Kit (AB273191)

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Cow

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.12 ng/mL

검출 범위

0.12 - 30 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Bovine IFN-alpha ELISA Kit (ab273191) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IFN-alpha in serum (bovine IFN-alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Bovine IFN-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IFN-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "108 - 134 %", "average": "= 120.6" }, { "sample": "Plasma", "range": "99 - 147 %", "average": "= 118.8" }, { "sample": "Cell culture media", "range": "123 - 149 %", "average": "= 135.3" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273191-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273191-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273191-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273191-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273191-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Bovine IFN-alpha coated Microplate (12 x 8 wells)", "number":"AB273191-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB273191-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Bovine IFN-alpha Standard (Lyophilized)", "number":"AB273191-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Bovine IFN-alpha Antibody", "number":"AB273191-CMP07", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information IFNA1_BOVIN
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websiteProtocolBooklet
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Product promise

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