JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325037

Caffeine ELISA Kit - Extracellular

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Caffeine ELISA Kit - Extracellular is a Competitive ELISA for the measurement of Caffeine - Extracellular in in Biofluids, Cell Culture Media samples.
7 이미지
Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Interpolated concentration of native Caffeine was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 2,000 Human serum, and 1 : 4,000 Human plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Cell Extraction Buffer SSW.

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Example of ​Caffeine​ standard curve in Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Free Caffiene was spiked into the following Mouse samples and diluted in a 2-fold dilution series in Cell Extraction Buffer SSW. Undiluted samples are 25% Mouse serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Free Caffeine was spiked into the following Rat samples and diluted in a 2-fold dilution series in Cell Extraction Buffer SSW. Undiluted samples are 25% Rat serum, plasma (citrate), plasma (EDTA) and plasma (heparin) The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Free Caffeine was spiked into the following samples and diluted in a 2-fold dilution series in Cell Extraction Buffer SSW. Undiluted samples are as follows : Human low serum at 25% and cell culture media at 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Interpolated concentration of native Caffeine was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 10,000 Human saliva and 1 : 20,000 Human urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Cell Extraction Buffer SSW.

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)
  • sELISA

Lab

Sandwich ELISA - Caffeine ELISA Kit - Extracellular (AB325037)

Serum of ten individual healthy Species male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Caffeine concentration was determined to be 98.5 ng/mL with a range of 0.2 – 355.6 ng/mL.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cell culture supernatant

분석 유형

Competitive

결과 유형

Qualitative

Sensitivity

= 2.391 pg/mL

검출 범위

9.375 - 600 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Primary Antibodies

AB218230

Anti-beta 2 Microglobulin antibody [EPR21752-214]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EPR21752-214] (AB218230)

  • 4
  • 2
Reagents, Controls & Accessories

AB206978

Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA®

SimpleStep

ELISA - Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA® (AB206978)

  • 0
  • 0
Primary Antibodies

AB300457

Anti-TMEM175 antibody [EPR24415-47]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM175 antibody [EPR24415-47] (AB300457)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Caffeine ELISA Kit- Extracellular (ab325037) is a rapid single-wash competitive 90-min ELISA to measure Caffeine in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cell Culture Supernatant. This SimpleStep sensitivity is 2.391 pg/ml.<br><br><strong>How the assay works</strong><br><br>Caffeine ELISA Kit- Extracellular (ab325037) uses an affinity tag recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA® allows the formulation of the antibody-analyte complex in a single step, saving you significant time at the bench.<br>In the antigen-down format, the labeled antigen and the unlabeled antigen from your sample compete to bind to the HRP-tagged capture antibody. In the antibody-down format, the HRP-tagged antigen and the unlabeled antigen from your sample compete to bind to the capture antibody. The component labeled with the CaptSure™ affinity tag, either antigen or antibody, binds to the SimpleStep ELISA® plate. A decrease in signal indicates the presence of antigen in the sample when compared to wells containing only the labeled antigen.<br><br><strong>Assay Specificity</strong><br><br>Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.<br>Please refer to our protocol booklet for more details.<br><br><strong>Caffeine (ab325037) protocol summary</strong><br><br>1. Mix : add samples / standards to the wells together with the Captsure conjugate solution and HRP Conjugate solution. Incubate 1 hr at room temperature<br>2. Wash<br>3. Add TMB development solution - incubate for 10 min<br>4. Add Stop solution<br>5. Read the results on a plate reader at 450 nm<br><br>

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "87 - 115 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "98 - 105 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "101 - 115 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "95 - 102 %", "average": "= 97" }, { "sample": "Saliva", "range": "100 - 102 %", "average": "= 101" }, { "sample": "Urine", "range": "102 - 110 %", "average": "= 105" }, { "sample": "Cell culture supernatant", "range": "95 - 104 %", "average": "= 99" } ]
style
left-column

제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab325037-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB325037-CMP11" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325037-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB325037-CMP10" }, { "size":"10 x 80 µL", "name":"50X Caffeine HRP Conjugate", "number":"AB325037-CMP02" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325037-CMP06" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB325037-CMP09" }, { "size":"20 x 1 Vial", "name":"Caffeine Lyophilized Standard", "number":"AB325037-CMP03" }, { "size":"10 x 1 Vial", "name":"Lyophilized Caffeine CaptSure Conjugate", "number":"AB325037-CMP01" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB325037-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB325037-CMP08" }, { "size":"10 x 8 mL", "name":"Antibody Diluent 5BI", "number":"AB325037-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325037-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB325037-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Cell Extraction Buffer SSW", "number":"AB325037-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent 5BI", "number":"AB325037-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB325037-CMP08", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Caffeine HRP Conjugate", "number":"AB325037-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB325037-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Caffeine Lyophilized Standard", "number":"AB325037-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Caffeine CaptSure Conjugate", "number":"AB325037-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB325037-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325037-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325037-CMP07", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

See full target information Caffeine

대체 명칭 보기

A1AR, A1R, A3AR, A3R, AA1R_HUMAN, AA3R_HUMAN, ACEE, ACES_HUMAN, ACLS, AD 026, ADORA 1, ADORA 3, AIS, ANDR_HUMAN, AR, AR8, ARA3, ARACHE, Acetylcholinesterase, Adenosine A3 receptor, Adenosine receptor A1, Adenosine receptor A3, Androgen nuclear receptor variant 2, Androgen receptor, Androgen receptor (dihydrotestosterone receptor, testicular feminization, spinal and bulbar muscular atrophy, Kennedy disease), Apoptosis related acetylcholinesterase, Atherosclerosis, susceptibility to, included, BXR, DHTR, DKFZp686N23123, DNA binding protein, Dihydro testosterone receptor, Dihydrotestosterone receptor (DHTR), ER, ER-alpha, ER-beta, ERR a, ERR-alpha, ERR1 protein, ERR1_HUMAN, ER[a], ER[b], ESR, ESR B, ESR BETA, ESR1_HUMAN, ESRA, ESRL 1, ESRR A, ESTR B, Eag-related protein 1, Era, Erb, Erb2, Estr, Estra, Estradiol Receptor alpha, Estradiol Receptor beta, Estradiol receptor, Estrogen Receptor 1, Estrogen Receptor 2, Estrogen receptor, Estrogen receptor 1 (alpha), Estrogen receptor 2 (ER beta), Estrogen receptor 2 ER beta, Estrogen receptor alpha, Estrogen receptor beta 4, Estrogen receptor related 1, Estrogen receptor-like 1, Estrogen resistance, included, Estrogen-related receptor alpha, Estrra, Ether a go go related potassium channel protein, Ether-a-go-go-related gene potassium channel 1, Ether-a-go-go-related protein 1, GCPS, GLI Kruppel family member GLI 3, GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome), GLI family zinc finger 3, GLI3 C-terminally truncated form, GLI3 form of 190 kDa, GLI3 form of 83 kDa, GLI3 full length protein, GLI3-190, GLI3-83, GLI3FL, GLI3_HUMAN, GPCR 2, Glioma associated oncogene family zinc finger 3, H-ERG, HDL cholesterol, augmented response of, to hormone replacement, included, HUMARA, HYSP1, IMD42, KCNH2_HUMAN, KD, Kennedy disease (KD), Kv11.1, LQT 2, MGC129539, Myocardial infarction, susceptibility to, included, N-ACHE, NR1F3, NR1I2_HUMAN, NR3A1, NR3A2, NR3B1, NR3C4, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Nuclear receptor subfamily 1 group I member 2, Nuclear receptor subfamily 3 group A member 1, Nuclear receptor subfamily 3 group A member 2, Nuclear receptor subfamily 3 group B member 1, Nuclear receptor subfamily 3 group C member 4, Nuclear receptor subfamily 3 group C member 4 (NR3C4), ONR 1, OTTHUMP00000017718, OTTHUMP00000017719, OTTHUMP00000215173, OTTHUMP00000215174, OTTHUMP00000215175, Oncogene GLI3, Orphan nuclear receptor PAR 1, Orphan nuclear receptor PXR, PAP A, PAPB, PAR, PAR q, PPD IV, PRR, Potassium channel HERG, Potassium voltage gated channel subfamily H (eag related) member 2, Potassium voltage-gated channel subfamily H member 2, Pregnane X receptor, RAR related orphan nuclear receptor variant 2, RAR related orphan receptor C, isoform a, RAR related orphan receptor gamma, RAR-related orphan receptor C, RDC 7, RNESTROR, RORG_HUMAN, RP11 552M11.7, RZR GAMMA, RZRG, Retinoic acid binding receptor gamma, Retinoid-related orphan receptor-gamma, Ri, Rorc, SBMA, SMAX1, SQT1, SXR, Spinal and bulbar muscular atrophy, Spinal and bulbar muscular atrophy (SBMA), Steroid and xenobiotic receptor, Steroid hormone receptor ERR1, TFM, TGPCR1, TOR, Testicular Feminization (TFM), Transcriptional activator GLI3, Transcriptional repressor GLI3R, Voltage gated potassium channel, subfamily H, member 2, Voltage-gated potassium channel subunit Kv11.1, YT, YT blood group, Zinc finger protein GLI 3, androgen receptor splice variant 4b, bA552M11.5, eag homolog, estrogen receptor related receptor alpha, hERG-1, hERR1, pregnane X nuclear receptor variant 2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com