AB290713

cAMP Assay Kit (Competitive ELISA)

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cAMP Assay Kit (Competitive ELISA) is a competitive ELISA designed to quantify cAMP.

- Colorimetric competitive ELISA - 405 nm readout - works on any plate reader
- Suitable for cells and tissue treated with 0.1M HCL, plasma, saliva, serum and cell culture supernatant.

4 이미지

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

cELISA

Supplier Data

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

cAMP Assay Kit (Competitive ELISA).

Representative non-acetylated standard curve generated using ab290713.

Assay Buffer used as diluent.

cELISA

Supplier Data

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

cAMP Assay Kit (Competitive ELISA).

Representative non-acetylated standard curve generated using ab290713.

0.1 M HCl used as diluent.

cELISA

Supplier Data

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

cAMP Assay Kit (Competitive ELISA).

Representative acetylated standard curve generated using ab290713.

Assay Buffer used as diluent.

cELISA

Supplier Data

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

cAMP Assay Kit (Competitive ELISA).

Representative acetylated standard curve generated using ab290713.

0.1 M HCl used as diluent.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Lysate, Saliva, Cell culture media, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Mammals

분석 유형

Competitive

분석 소요 시간

분석 플랫폼

Microplate

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제품 세부 정보

cAMP Assay Kit (Competitive ELISA) ab290713 is a competitive ELISA to measure cAMP in cells and tissue treated with 0.1M HCL, plasma, saliva, serum and cell culture supernatant..

How the assay works:

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cAMP antigen and a polyclonal rabbit antibody specific to cAMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cAMP captured in the plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

cAMP Assay Kit (Competitive ELISA) ab290713 protocol summary

1. Add standards and samples to appropriate wells
2. Add prepared labeled AP-conjugate to appropriate wells
3. Add Cyclic AMP Complete antibody to appropriate wells
4. Incubate at room temperature
5. Add pNpp substrate to each well. Incubate at room temperature
6. Add Stop Solution to each well. Read immediately.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

Multi

적절한 장기 보관 조건

Multi

보관 정보

Please refer to protocols

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cyclic adenosine monophosphate (cAMP) serves as an essential secondary messenger in various signal transduction pathways. Known also by its chemical abbreviation cAMP this small molecule has a mass of approximately 329.21 g/mol. cAMP is synthesized from ATP by the enzyme adenylate cyclase which is present in many cell types notably in neurons and various endocrine tissues. cAMP acts by activating protein kinase A (PKA) which then phosphorylates target proteins to alter cellular function.

Biological function summary

CAMP regulates numerous cellular processes including metabolism gene expression and cell growth. It functions as an important part of G protein-coupled receptor (GPCR) signaling pathways where its concentration can significantly change influenced by external signals. cAMP modulates the activity of ion channels and various enzymes often participating in feedback loops to maintain cellular homeostasis.

Pathways

CAMP integrates into critical signal transduction pathways such as the cyclic AMP-dependent pathway and the calcium signaling pathway. It works closely with proteins like CREB (cAMP response element-binding protein) to regulate gene transcription. cAMP also impacts other molecules like phosphodiesterases which break it down maintaining its transient nature in response to stimuli.

Irregular cAMP levels associate with pathologies like heart failure and certain types of cancer. Disruptions in cAMP signaling can lead to abnormal cell growth or inadequate cellular response to hormonal signals. Through its interaction with proteins like CFTR (cystic fibrosis transmembrane conductance regulator) cAMP influences disorders such as cystic fibrosis highlighting its critical role in maintaining cellular function and signaling balance.

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제품 프로토콜

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타겟 정보

See full target information cAMP

대체 명칭 보기

3'-5'-cyclic adenosine monophosphate, Adenosine 3' 5' cyclic monophosphate, Adenosine 3閳 5閳 cyclic monophosphate, Cyclic adenosine monophosphate

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Infection and immunity 93:e0027124 PubMed39841046

2025

MASP1 modulation as a novel therapeutic target in severe pediatric pertussis: insights from a multi-omics approach.

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Lin Xu,Caiying Wang,Yuhuan Liu,Yanlan Zhang,Zhen Li,Lin Pang

Food & function 15:11047-11059 PubMed39422021

2024

The quercetin metabolite 4-methylcatechol causes vasodilation voltage-gated potassium (K) channels.

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Patrícia Dias,Rudy Salam,Jana Pourová,Marie Vopršalová,Lukáš Konečný,Eduard Jirkovský,Jurjen Duintjer Tebbens,Přemysl Mladěnka

Cancer research 84:2626-2644 PubMed38888469

2024

Increased Protein Kinase A Activity Induces Fibrolamellar Hepatocellular Carcinoma Features Independent of DNAJB1.

Applications

Unspecified application

Species

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Mahsa Shirani,Solomon Levin,Bassem Shebl,David Requena,Tova M Finkelstein,Daniel S Johnson,Denise Ng,Gadi Lalazar,Søren Heissel,Peter Hojrup,Henrik Molina,Ype P de Jong,Charles M Rice,Aatur D Singhi,Michael S Torbenson,Philip Coffino,Barbara Lyons,Sanford M Simon

Journal of gastroenterology and hepatology 39:1413-1421 PubMed38348885

2024

Cilostazol improves the prognosis after hepatectomy in rats with sinusoidal obstruction syndrome.

Applications

Unspecified application

Species

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Hiroaki Sugita,Shinichi Nakanuma,Seiichi Munesue,Tatsuya Ishikawa,Tomokazu Tokoro,Ryohei Takei,Mitsuyoshi Okazaki,Kaichiro Kato,Satoshi Takada,Isamu Makino,Noriyuki Ozaki,Yasuhiko Yamamoto,Shintaro Yagi

Nature communications 14:4494 PubMed37524709

2023

Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34.

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Unspecified application

Species

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Ryuta Sugihara,Manabu Taneike,Tomokazu Murakawa,Takahito Tamai,Hiromichi Ueda,Rika Kitazume-Taneike,Takafumi Oka,Yasuhiro Akazawa,Hiroki Nishida,Kentaro Mine,Ayana Hioki,Jumpei Omi,Shigemiki Omiya,Junken Aoki,Kazutaka Ikeda,Kazuhiko Nishida,Makoto Arita,Osamu Yamaguchi,Yasushi Sakata,Kinya Otsu

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

cAMP Assay Kit (Competitive ELISA)

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

분석 소요 시간

분석 플랫폼

보완 제품

Secondary Antibodies

Secondary Antibodies

Assay Kits

Assay Kits

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise