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AB273185

Canine IFN-alpha ELISA Kit

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Canine IFN-alpha ELISA Kit is a Sandwich ELISA for the measurement of Canine IFN-alpha in Dog in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Canine IFN-alpha ELISA Kit (AB273185)
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Supplier Data

Sandwich ELISA - Canine IFN-alpha ELISA Kit (AB273185)

Canine IFN-alpha ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Dog

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.18 ng/mL

검출 범위

0.164 - 40 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Canine IFN-alpha ELISA Kit (ab273185) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IFN-alpha in serum (Canine IFN-alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA as an anti-coagulant. Citrate and heparin are not recommended as anti-coagulants for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Canine IFN-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IFN-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IFN-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IFN-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 109 %", "average": "= 100.9" }, { "sample": "Cell culture media", "range": "84 - 104 %", "average": "= 95.38" }, { "sample": "Plasma", "range": "91 - 106 %", "average": "= 98.4" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273185-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273185-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273185-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273185-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273185-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine IFN-alpha coated Microplate (12 x 8 wells)", "number":"AB273185-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273185-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine IFN-alpha Antibody", "number":"AB273185-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine IFN-alpha Standard (Lyophilized)", "number":"AB273185-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information Interferon alpha-1/2

대체 명칭 보기

Interferon alpha-1/2, IFN-alpha-1/2
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websiteProtocolBooklet
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Product promise

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