AB273170
Canine IL-1beta ELISA Kit
Canine IL-1beta ELISA 키트
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Canine IL-1beta ELISA Kit is a Sandwich ELISA for the measurement of Canine IL-1beta in Dog in Biofluids, Cell Culture Media samples.
1 이미지
- sELISA
Supplier Data
Sandwich ELISA - Canine IL-1beta ELISA Kit (AB273170)
caninine IL-1beta ELISA Standard Curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
보완 제품
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
제품 세부 정보
Canine IL-1beta ELISA Kit (ab273170) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine IL-1 beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for canine IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-canine IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This assay employs an antibody specific for canine IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-canine IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
Recovery
[ { "sample": "Serum", "range": "74 - 98 %", "average": "= 86.02" }, { "sample": "Plasma", "range": "68 - 107 %", "average": "= 84.08" }, { "sample": "Cell culture media", "range": "109 - 145 %", "average": "= 126.1" } ]
제품 구성
{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273170-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273170-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273170-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273170-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273170-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine IL-1beta coated Microplate (12 x 8 wells)", "number":"AB273170-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB273170-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine IL-1beta Standard (Lyophilized)", "number":"AB273170-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine IL-1beta Antibody", "number":"AB273170-CMP08", "productcode":"" } ] } } }
특성 및 보관 정보
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
타겟 정보
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
대체 명칭 보기
Interleukin-1 beta, IL-1 beta, IL1B
제품이 사용된 논문 (1)
Recent publications for all applications. Explore the 전체 목록 and refine your search
International journal of molecular sciences 24: PubMed37834419
2023
Effects of SKCPT on Osteoarthritis in Beagle Meniscectomy and Cranial Cruciate Ligament Transection Models.
Applications
Unspecified application
Species
Unspecified reactive species
Hye-Min Kim,Minseok Kang,Yoon-Seok Jung,Yoon-Jung Lee,Wonjae Choi,Hunseung Yoo,JeongHoon Kim,Hyo-Jin An
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