- sELISA
Supplier Data
Sandwich ELISA - Canine RAGE ELISA Kit (AB273182)
Canine RAGE ELISA Kit standard curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
보완 제품
Reactivity 정보
제품 세부 정보
Canine RAGE ELISA Kit (ab273182) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine RAGE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Precision
Recovery
제품 구성
특성 및 보관 정보
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RAGE functions in the immune and inflammatory response where it mediates cell signaling that leads to cellular activation and the release of pro-inflammatory cytokines. It acts as part of complexes with different proteins contributing to cellular processes such as proliferation and migration. RAGE also plays roles in the regulation of oxidative stress and apoptosis impacting cellular health and survival. Researchers employ tools like 'anti-RAGE' antibodies and 'RAGER ELISA' assays to measure and study RAGE expression levels and its interactions in various experimental setups.
Pathways
RAGE is significantly involved in the NF-kB pathway and the MAPK signaling cascade. Its activation can lead to the release of NF-kB a transcription factor that plays an essential role in immune and inflammatory responses. RAGE interacts with proteins such as p38 MAPK leading to a cascade of events that regulate inflammation and stress responses. The signaling pathways involving RAGE are important in maintaining cell homeostasis and responding to cellular stressors and tools like 'anti-RAGE' and 'mouse RAGE' antibodies serve to elucidate these complex pathways further.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com