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AB273180

Canine VEGFA ELISA Kit

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Canine VEGFA ELISA Kit is a Sandwich ELISA for the measurement of Canine VEGFA in Dog in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Canine VEGFA ELISA Kit (AB273180)
  • sELISA

Supplier Data

Sandwich ELISA - Canine VEGFA ELISA Kit (AB273180)

Canine VEGFA ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Dog

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 6 pg/mL

검출 범위

6.14 - 1500 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Canine VEGFA ELISA Kit (ab273180) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine VEGFA in serum (Canine VEGFA concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as anti-coagulants for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Canine VEGFA coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine VEGFA present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine VEGFA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine VEGFA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "67 - 104 %", "average": "= 81.89" }, { "sample": "Cell culture media", "range": "95 - 141 %", "average": "= 117.6" }, { "sample": "Plasma", "range": "68 - 76 %", "average": "= 71.22" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273180-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273180-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273180-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273180-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273180-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine VEGFA coated Microplate (12 x 8 wells)", "number":"AB273180-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273180-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine VEGFA Antibody", "number":"AB273180-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine VEGFA Standard (Lyophilized)", "number":"AB273180-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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제품 프로토콜

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타겟 정보

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).
See full target information VEGFA

대체 명칭 보기

VEGF, VEGFA, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF
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websiteProtocolBooklet
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Product promise

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