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AB273260

Chicken IFN-alpha ELISA Kit

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Chicken IFN-alpha ELISA Kit is a Sandwich ELISA for the measurement of Chicken IFN-alpha in Chicken in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Chicken IFN-alpha ELISA Kit (AB273260)
  • sELISA

Supplier Data

Sandwich ELISA - Chicken IFN-alpha ELISA Kit (AB273260)

Chicken IFN-alpha ELISA Standard Curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Chicken

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 22 pg/mL

검출 범위

20.48 - 5000 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Chicken IFN alpha ELISA Kit (ab273260) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IFN alpha in serum (Chicken IFN-alpha concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma EDTA (Citrate and Heparin have not been validated yet) and cell culture supernatants.

This assay employs an antibody specific for Chicken IFN alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IFN alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "73 - 134 %", "average": "= 104.9" }, { "sample": "Cell culture media", "range": "76 - 113 %", "average": "= 93.02" }, { "sample": "Plasma", "range": "67 - 106 %", "average": "= 84.98" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273260-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273260-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273260-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273260-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273260-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273260-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chicken IFN-alpha coated Microplate (12 x 8 wells)", "number":"AB273260-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Chicken IFN-alpha Antibody", "number":"AB273260-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Chicken IFN-alpha Standard (Lyophilized)", "number":"AB273260-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB273260-CMP07", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

See full target information IFN-alpha

대체 명칭 보기

IFL, IFN, IFN Alpha, IFNA13, IFNAP22, Interferon Alpha, Interferon alpha 1, Interferon alpha 1/13 precursor, Interferon alpha-D, LeIF D
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of animal physiology and animal nutrition 106:132-138 PubMed33964044

2021

Tetramethylpyrazine supplementation improves performance, digestion, blood and immune state of broilers exposure to oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyi Shi,Yiran Luo,Yufa Li,Feike Zhang,Ning Liu
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

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