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AB327286

Chicken IgY ELISA Kit, Chemiluminescent

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Chicken IgY ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Chicken IgY with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 36.58 - 80000 pg/ml with a sensitivity of 8.22 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders

- Patent pending
3 이미지
Sandwich ELISA - Chicken IgY ELISA Kit, Chemiluminescent (AB327286)
  • sELISA

Lab

Sandwich ELISA - Chicken IgY ELISA Kit, Chemiluminescent (AB327286)

Standard curve comparison between the Chemiluminescent Chicken Immunoglobulin Y SimpleStep ELISA and colorimetric Chicken Immunoglobulin Y SimpleStep ELISA (ab189577) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Chicken IgY ELISA Kit, Chemiluminescent (AB327286)
  • sELISA

Lab

Sandwich ELISA - Chicken IgY ELISA Kit, Chemiluminescent (AB327286)

Dynamic range comparison between the Chemiluminescent Chicken Immunoglobulin Y SimpleStep ELISA, colorimetric Chicken Immunoglobulin Y SimpleStep ELISA (ab189577), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Chicken IgY ELISA Kit, Chemiluminescent (AB327286)
  • sELISA

Lab

Sandwich ELISA - Chicken IgY ELISA Kit, Chemiluminescent (AB327286)

Interpolated concentration of native Immunoglobulin Y was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 10,000 mayonnaise. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

주요 정보

검출 방식

Luminescent

샘플 타입

10F DMEM Medium, 1X Cell Extraction Buffer PTR, 1X Wash Buffer, Antibody Diluent 4B, OF DMEM Medium, Mayonnaise

Reacts with

Chicken

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 8.22 pg/mL

검출 범위

36.58 - 80000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Chicken IgY ELISA Kit, Chemiluminescent (ab327286) is a rapid single-wash sandwich 55-min ELISA to measure Chicken IgY in 10F DMEM Medium, 1X Cell Extraction Buffer PTR, 1X Wash Buffer, Antibody Diluent 4B, OF DMEM Medium, Mayonnaise with a sensitivity of 8.22 pg/mL.

How the assay works

Chicken IgY ELISA Kit, Chemiluminescent (ab327286) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Chicken IgY (ab327286) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.49" } ]
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Recovery

[ { "sample": "10F DMEM Medium", "range": "70.17 - 86.63 %", "average": "= 75.89" }, { "sample": "1X Cell Extraction Buffer PTR", "range": "97.68 - 100.53 %", "average": "= 98.48" }, { "sample": "1X Wash Buffer", "range": "87.31 - 104.46 %", "average": "= 98.34" }, { "sample": "Antibody Diluent 4B", "range": "99.6 - 105.68 %", "average": "= 103.18" }, { "sample": "OF DMEM Medium", "range": "52.05 - 76.75 %", "average": "= 62.52" }, { "sample": "Mayonnaise", "range": "84 - 87 %", "average": "= 86" } ]
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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab327286-10x96Tests", "assetComponentDetails": [ { "size":"10 x 600 µL", "name":"10X Chicken IgY Detector Antibody", "number":"AB327286-CMP02" }, { "size":"10 x 600 µL", "name":"10X Chicken IgY Capture Antibody", "number":"AB327286-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327286-CMP11" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB327286-CMP12" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent A", "number":"AB327286-CMP09" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB327286-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4B", "number":"AB327286-CMP04" }, { "size":"10 x 1 Vial", "name":"Chicken IgY Lyophilized Purified Protein", "number":"AB327286-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327286-CMP08" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent B", "number":"AB327286-CMP10" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327286-CMP07" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327286-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab327286-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Chicken IgY Capture Antibody", "number":"AB327286-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Chicken IgY Detector Antibody", "number":"AB327286-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327286-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4B", "number":"AB327286-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Chicken IgY Lyophilized Purified Protein", "number":"AB327286-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB327286-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327286-CMP11", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB327286-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB327286-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327286-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB327286-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327286-CMP08", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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