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AB273259

Chicken IL-10 ELISA Kit

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Chicken IL-10 ELISA Kit is a Sandwich ELISA for the measurement of Chicken IL-10 in Chicken in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Chicken IL-10 ELISA Kit (AB273259)
  • sELISA

Supplier Data

Sandwich ELISA - Chicken IL-10 ELISA Kit (AB273259)

Chicken IL-10 ELISA Kit standard curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Chicken

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 16 pg/mL

검출 범위

14.34 - 3500 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Chicken IL-10 ELISA Kit (ab273259) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-10 in serum (Chicken IL-10 concentration is low in normal serum/plasma, and may not be detectable in this assay), EDTA plasma (Citrate and heparin have not been validated in this assay yet) and cell culture supernatants.

This assay employs an antibody specific for Chicken IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 143 %", "average": "= 105.8" }, { "sample": "Cell culture media", "range": "115 - 143 %", "average": "= 129.5" }, { "sample": "Plasma", "range": "70 - 105 %", "average": "= 86.13" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273259-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273259-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273259-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273259-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273259-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chicken IL-10 coated Microplate (12 x 8 wells)", "number":"AB273259-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Chicken IL-10 Standard (Lyophilized)", "number":"AB273259-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Chicken IL-10 Antibody", "number":"AB273259-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB273259-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10

대체 명칭 보기

Interleukin-10, IL-10, IL10
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The veterinary quarterly 44:1-20 PubMed38961536

2024

Insights of early feeding regime supplemented with glutamine and various levels of omega-3 in broiler chickens: growth performance, muscle building, antioxidant capacity, intestinal barriers health and defense against mixed infection.

Applications

Unspecified application

Species

Unspecified reactive species

Asmaa T Y Kishawy,Reham A Abd El-Wahab,Fatma Eldemery,Mona Mohammed I Abdel Rahman,Saleh Altuwaijri,Rasha M M Ezz-Eldin,Ehab M Abd-Allah,Shimaa Zayed,Zohair S Mulla,Rasha B El Sharkawy,Shereen Badr,Wessam Youssef,Doaa Ibrahim
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

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