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AB324665

Cotinine ELISA Kit - Extracellular

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Cotinine ELISA Kit - Extracellular is a Competitive ELISA for the measurement of Cotinine - Extracellular in in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Cotinine concentration was determined to be 98.5 ng/mL with a range of 0.2 – 355.6 ng/mL.

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)

Free ​Cotinine​ was spiked into the following Mouse samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% Mouse serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)

Free Cotinine was spiked into the following Rat samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% Rat serum, plasma (citrate), plasma (EDTA) and plasma (heparin) The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)

Interpolated concentration of native ​Cotinine​ was measured in duplicate at different sample concentrations. Undiluted samples are 1% Human serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)

Example of ​Cotinine​ standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Extracellular (AB324665)

Free ​Cotinine​ was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows : Human Non-Smoker serum 25%, urine 50%, saliva 50%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cell culture supernatant

분석 유형

Competitive

결과 유형

Quantitative

Sensitivity

= 22.793 pg/mL

검출 범위

46.875 - 3000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Cotinine ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Cotinine with a sensitivity of 22.793pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric competitive ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders

-A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.8" }, { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.7" } ]
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Recovery

[ { "sample": "Serum", "range": "104 - 114 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "104 - 118 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "98 - 107 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "104 - 114 %", "average": "= 108" }, { "sample": "Saliva", "range": "97 - 99 %", "average": "= 98" }, { "sample": "Urine", "range": "99 - 100 %", "average": "= 99" }, { "sample": "Cell culture supernatant", "range": "102 - 107 %", "average": "= 104" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

See full target information Cotinine
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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