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AB324664

Cotinine ELISA Kit - Intracellular

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Cotinine ELISA Kit - Intracellular is a Competitive ELISA for the measurement of Cotinine - Intracellular in in Cell/Tissue Extracts samples.
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Sandwich ELISA - Cotinine ELISA Kit - Intracellular (AB324664)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Intracellular (AB324664)

Example of ​Cotinine​ standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Cotinine ELISA Kit - Intracellular (AB324664)
  • sELISA

Lab

Sandwich ELISA - Cotinine ELISA Kit - Intracellular (AB324664)

Free ​Cotinine​ was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are 500 µg/mL A549 cell extract, HeLa cell extract, Jurkat cell extract, and Human lung extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Extract, Tissue Extracts

분석 유형

Competitive

결과 유형

Quantitative

Sensitivity

= 18.804 pg/mL

검출 범위

46.875 - 3000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Cotinine ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Cotinine with a sensitivity of 18.804pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric competitive ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders

-A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Tissue Extracts", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.8" }, { "reproducibilityType": "Inter", "sample": "Tissue Extracts", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "95 - 101 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "92 - 104 %", "average": "= 98" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

See full target information Cotinine
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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