Exendin-4 ELISA Kit
Exendin-4 ELISA 키트
- SimpleStep
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- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab325624
- ELISA
Supplier Data
ELISA - Exendin-4 ELISA Kit (AB272192)
- sELISA
Supplier Data
Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)
Example of cell culture media Exendin-4 standard curve in Sample Diluent NS.
The Exendin-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)
Interpolated concentrations of spiked Exendin-4 RPMI-1640 cell culture media +/- 10% FBS samples.
- sELISA
Lab
Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)
Example of Exendin-4 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Exendin-4 ELISA Kit (AB272192)
Recombinant Exendin-4 was spiked into the following species samples and diluted in a 2-fold dilution series in Sample Diluent NS in 96-well vs. 384-well plates. Undiluted samples are 25% cell culture media (RPMI containing 10% FBS). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
보완 제품
Reactivity 정보
제품 세부 정보
Exendin-4 ELISA Kit ab272192 is a rapid single-wash 90-min Sandwich ELISA to measure Exendin-4 in cell culture media. This SimpleStep sensitivity is 2.58 pg/mL.
How the assay works
Exendin-4 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Exendin-4 ELISA Kit ab272192 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Exendin-4 is a 39 amino acid peptide found in venom from the Gila monster Helicoderma suspectum. Exendin-4 is a member of the glucagon secretin family of peptide hormones and neuropeptides. The venom protein mimics the incretin hormone glucagon-like peptide 1 (GLP-1) and stimulates insulin synthesis and secretion, protects against beta-cell apoptosis in response to different insults, and promotes beta-cell proliferation. Exendin-4 also promotes satiety, reduces food intake, fat deposition and body weight. It also is known to inhibit gastric emptying. Exendin-4 interactions with GLP-1 receptor (GLP1R). induces hypotension that is mediated by relaxation of cardiac smooth muscle.
Precision & recovery 정보
Precision
[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]
Recovery
[ { "sample": "Cell culture media", "range": "113 - 118 %", "average": "= 115" } ]
제품 구성
특성 및 보관 정보
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보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Exendin-4 enhances insulin secretion helping to regulate blood glucose levels effectively. It mimics the activity of GLP-1 which is involved in stimulating insulin release. Exendin-4 also slows gastric emptying and promotes satiety contributing to reduced food intake. Although the peptide does not form part of a larger complex its interactions directly affect multiple cellular processes relevant to metabolism.
Pathways
Exendin-4 participates in the insulin signaling and glucose homeostasis pathways. It influences insulin receptor substrate proteins thereby playing an important role in maintaining normal blood sugar levels. Exendin-4 modulates the activity of gluconeogenic enzymes connecting it to metabolic pathways involving energy balance and storage.
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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